Tian Tian, Zhang Enpei, Fei Fei, Li Xiaofei, Guo Xu, Liu Boya, Li Jibin, Chen Zhinan, Xing Jinliang
College of Life Science and Bioengineering, Beijing Jiaotong University, Beijing, PR China.
Asian Pac J Cancer Prev. 2011;12(8):2039-43.
Lung cancer is one of the most common cancer types that lead to death in cancer patients across the world. Clarification of differentially expressed genes is essential for lung cancer diagnosis and treatment. According to the public cDNA microarray database, we found that GTSE1 is overexpressed dramatically in lung cancer patients' tissues. To further verify the microarray data, quantitative real-time PCR and immunohistochemical staining was undertaken to test GTSE1 expression both at mRNA and protein levels. GTSE1 is up-regulated in lung cancer tissues compared to the adjacent normal tissues, especially in adenocarcinoma and squamous cell carcinoma. The expression profile of GTSE1 was investigated, and the clinical significance and the association between GTSE1 and the overall survival assessed in 246 lung cancer patients. No statistically significant correlation, however, was observed between GTSE1, the clinical features and survival. Thus, GTSE1 may not be a prognostic marker in lung cancers.
肺癌是导致全球癌症患者死亡的最常见癌症类型之一。阐明差异表达基因对于肺癌的诊断和治疗至关重要。根据公共cDNA微阵列数据库，我们发现GTSE1在肺癌患者组织中显著过表达。为了进一步验证微阵列数据，进行了定量实时PCR和免疫组织化学染色，以检测GTSE1在mRNA和蛋白质水平的表达。与相邻正常组织相比，GTSE1在肺癌组织中上调，尤其是在腺癌和鳞状细胞癌中。研究了GTSE1的表达谱，并在246例肺癌患者中评估了GTSE1的临床意义及其与总生存期的关系。然而，未观察到GTSE1与临床特征及生存期之间存在统计学上的显著相关性。因此，GTSE1可能不是肺癌的预后标志物。
