Department of Zoology, University of Delhi, Delhi 110007, India.
Infect Genet Evol. 2012 Mar;12(2):411-9. doi: 10.1016/j.meegid.2012.01.015. Epub 2012 Jan 24.
Bemisia tabaci is the major vector pest of agricultural crops all over the world. In this study we report the different bacterial endosymbionts associated with B. tabaci sampled from 14 different locations in North India. Using 16S rDNA clone library sequences we were able to identify Portiera, the primary endosymbiont of B. tabaci, and other secondary endosymbionts like Cardinium, Wolbachia, Rickettsia and Arsenophonus. Along with these we also detected Bacillus, Enterobacter, Paracoccus and Acinetobacter. These secondary endosymbionts were not uniformly distributed in all the locations. Phylogenetic analysis of 16S rDNA sequences of Cardinium, Wolbachia, Rickettsia and Arsenophonus showed that each of these bacteria form a separate cluster when compared to their respective counterparts from other parts of the world. MtCO1 gene based phylogenetic analysis showed the presence of Asia I and Asia II genetic groups of B. tabaci in N. India. The multiple correspondence analyses showed no correlation between the host genetic group and the endosymbiont diversity. These results suggest that the bacterial endosymbiont diversity of B. tabaci is much larger and complex than previously perceived and probably N. Indian strains of the bacterial symbionts could have evolved from some other ancestor.
烟粉虱是全世界农业作物的主要媒介害虫。在本研究中，我们报告了从印度北部 14 个不同地点采集的烟粉虱所携带的不同细菌共生体。通过 16S rDNA 克隆文库序列，我们能够鉴定出烟粉虱的主要共生菌 Portiera，以及其他次要共生菌，如卡多体菌、沃尔巴克氏体、立克次氏体和放线菌。除了这些，我们还检测到了芽孢杆菌、肠杆菌、副球菌和不动杆菌。这些次要共生菌并非在所有地点都均匀分布。卡多体菌、沃尔巴克氏体、立克次氏体和放线菌的 16S rDNA 序列的系统发育分析表明，与来自世界其他地区的相应细菌相比，它们各自形成一个单独的聚类。基于 MtCO1 基因的系统发育分析显示，在印度北部存在亚洲 I 和亚洲 II 遗传组的烟粉虱。多重对应分析表明，宿主遗传组与共生体多样性之间没有相关性。这些结果表明，烟粉虱的细菌共生体多样性比之前认为的要大得多且复杂得多，而且印度北部的细菌共生体菌株可能是从其他一些祖先进化而来的。
