Haynes K A, Latge J P, Rogers T R
Department of Medical Microbiology, Charing Cross and Westminister Medical School, London United Kingdom.
J Clin Microbiol. 1990 Sep;28(9):2040-4. doi: 10.1128/jcm.28.9.2040-2044.1990.
Serial urine samples were collected from 33 neutropenic patients, 10 of whom developed invasive aspergillosis (IA) while undergoing bone marrow transplantation or remission induction therapy for leukemia. Concentrated urine samples from the infected patients were subjected to polyacrylamide gel electrophoresis, blotted, and then incubated with antiserum raised to a cell wall extract of Aspergillus fumigatus (anti-CW) or an immunoglobulin G monoclonal antibody to A. fumigatus galactomannan (EBA1). When IA patient urine blots were probed with anti-CW, major bands at 11 and 18 kilodaltons (kDa); intermediate bands at 13, 14, and 29 kDa; and minor bands at 38 and 44 kDa were seen. In contrast, EBA1 showed diffuse staining at molecular masses larger than 45 kDa and a single weak band at 21 kDa. Urine samples from the 23 patients with no evidence of IA were unreactive with both anti-CW and EBA1. These antigen bands are likely to represent immunodominant antigens which are excreted during IA and should play a valuable role in the development of rapid diagnostic tests for aspergillosis.
从33例中性粒细胞减少患者中收集系列尿样，其中10例在接受骨髓移植或白血病缓解诱导治疗期间发生了侵袭性曲霉病（IA）。对感染患者的浓缩尿样进行聚丙烯酰胺凝胶电泳、印迹，然后与针对烟曲霉细胞壁提取物产生的抗血清（抗CW）或抗烟曲霉半乳甘露聚糖的免疫球蛋白G单克隆抗体（EBA1）孵育。用抗CW探测IA患者尿印迹时，可见11和18千道尔顿（kDa）处的主要条带、13、14和29 kDa处的中间条带以及38和44 kDa处的次要条带。相比之下，EBA1在大于45 kDa的分子量处显示弥漫性染色，在21 kDa处有一条单一的弱条带。23例无IA证据患者的尿样与抗CW和EBA1均无反应。这些抗原条带可能代表IA期间排泄的免疫显性抗原，在曲霉病快速诊断试验的开发中应发挥重要作用。
