Department of Pathology and Biomedical Science, University of Otago, Christchurch 8011, New Zealand.
Canterbury Health Laboratories, Christchurch 8011, New Zealand.
Int J Mol Sci. 2021 Dec 27;23(1):252. doi: 10.3390/ijms23010252.
Invasive aspergillosis (IA) is a life-threatening disease mainly caused by and . Early diagnosis of this condition is crucial for patient treatment and survival. As current diagnostic techniques for IA lack sufficient accuracy, we have raised two monoclonal antibodies (1D2 and 4E4) against cell wall fragments that may provide a platform for a new diagnostic approach. The immunoreactivity of these antibodies was tested by immunofluorescence and ELISA against various and species in vitro and by immunohistochemistry in infected mouse tissues. Both monoclonal antibodies (mAbs) showed intensive fluorescence with the hyphae wall of and , but there was no staining with other species or species. Both mAbs also showed strong immunoreactivity to the cell wall of hyphae in the infected liver, spleen and kidney of mice with IA. The antigens identified by 1D2 and 4E4 might be glycoproteins and the epitopes are most likely a protein or peptide rather than a carbohydrate. An antibody-based antigen capture ELISA detected the extracellular antigens released by , , and , but not in species. The antigen could be detected in the plasma of mice after 48 h of infection by double-sandwich ELISA. In conclusion, both 1D2 and 4E4 mAbs are potentially promising diagnostic tools to investigate invasive aspergillosis.
侵袭性曲霉病（IA）是一种危及生命的疾病，主要由 和 引起。这种疾病的早期诊断对患者的治疗和生存至关重要。由于目前 IA 的诊断技术准确性不足，我们已经提出了两种针对 细胞壁片段的单克隆抗体（1D2 和 4E4），它们可能为新的诊断方法提供平台。通过免疫荧光和 ELISA 对体外各种 和 种进行了这两种单克隆抗体（mAbs）的免疫反应性测试，并用免疫组织化学方法对感染了 的小鼠组织进行了测试。两种单克隆抗体均与 和 的菌丝壁表现出强烈的荧光反应，但与其他 种或 种无染色反应。两种 mAb 对 IA 感染的小鼠肝脏、脾脏和肾脏中的菌丝细胞壁也表现出强烈的免疫反应。1D2 和 4E4 识别的抗原可能是糖蛋白，表位很可能是蛋白质或肽，而不是碳水化合物。基于抗体的抗原捕获 ELISA 检测到了 、 、 和 释放的细胞外抗原，但在 种中没有检测到。双夹心 ELISA 可在感染后 48 h 检测到小鼠血浆中的抗原。总之，1D2 和 4E4 mAb 是一种很有前途的诊断侵袭性曲霉病的工具。
