Moore P M
Department of Neurology, Wayne State University School of Medicine, Detroit, MI 48202.
J Neuroimmunol. 1990 Dec;30(2-3):101-9. doi: 10.1016/0165-5728(90)90093-3.
Screening serum by enzyme-linked immunosorbent assay (ELISA) to paraformaldehyde-fixed neuroblastoma cells revealed spontaneous neuron-reactive antibodies in three strains of autoimmune mice not present in comparable studies of BALB/c mice. Immunoglobulin isolation from pooled sera by either ammonium sulfate precipitation or passage over a protein G column enabled quantitative binding by (1) ELISA to neuroblastoma cells and (2) Western blots of plasma membrane preparations of brain cortex and neuroblastoma cells. The antibodies recognized proteins of apparent molecular weights 101,000, 68,000, 63,000, 57,000, 53,000, 43,000, 39,000, and 31,000 Da on the brain cortex and 63,000, 57,000, and 43,000 Da on the neuroblastoma cell membranes. The class of antibody binding was predominantly IgG in the MRL/lpr and IgM in the NZB/W. Differences between MRL/lpr, NZB/W and BXSB mice were observed although it is not yet apparent if this represents a difference in autoantibody production between the strains.
