Groenink Lucianne, Vinkers Christiaan, van Oorschot Ruud, Olivier Berend
Utrecht Institute for Pharmaceutical Sciences and Rudolf Magnus Institute of Neuroscience, Utrecht, The Netherlands.
Curr Protoc Pharmacol. 2009 Jun;Chapter 5:Unit 5.16. doi: 10.1002/0471141755.ph0516s45.
Described in this unit is the stress-induced hyperthermia (SIH) test in mice in a single-housed format. This protocol has proven reliable in detecting the anxiolytic properties of test compounds. In this test, SIH is quantified in singly housed mice using a rectal temperature measurement as the stressor. Rectal temperature is measured twice at a 10-min interval. Due to the stress experienced during the first temperature measurement, the temperature of the second measurement (T(2)) is ∼0.8° to 1.5°C higher than that of the first (T(1)). This difference in temperature (ΔT = T(2) - T(1)) is defined as the SIH response. The SIH response is reduced by different classes of anxiolytics. The SIH test is simple and robust, it does not require training of animals, and test compound effects on motor behavior, feeding, and nociception do not affect test outcome. Furthermore, it is one of few anxiety tests that focuses on the physiological component of anxiety.
本单元介绍了以单笼饲养形式对小鼠进行应激诱导性体温过高（SIH）测试的方法。该方案已被证明在检测受试化合物的抗焦虑特性方面是可靠的。在该测试中，通过测量直肠温度作为应激源，对单笼饲养的小鼠的SIH进行量化。直肠温度每隔10分钟测量两次。由于在第一次温度测量期间经历了应激，第二次测量的温度（T(2)）比第一次（T(1)）高约0.8°至1.5°C。这种温度差异（ΔT = T(2) - T(1)）被定义为SIH反应。不同类别的抗焦虑药会降低SIH反应。SIH测试简单且稳健，不需要对动物进行训练，受试化合物对运动行为、进食和痛觉感受的影响不会影响测试结果。此外，它是少数关注焦虑生理成分的焦虑测试之一。
