Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China.
Biotechnol Lett. 2012 May;34(5):925-33. doi: 10.1007/s10529-012-0853-3.
Paenibacillus polymyxa SQR-21, which is antagonistic against Fusarium oxysporum, is used as a biocontrol agent and, when mixed with organic substances for solid fermentation, produces a bioorganic fertilizer. The spores of P. polymyxa prepared at different temperatures were characterized with respect to the dipicolinic acid content, heat resistance, fatty acid composition and germination. Spores prepared at 37°C showed higher heat resistance than those prepared at 25 and 30°C. However, the germination rate was negatively correlated with the sporulation temperature. The maximum germination rate of the spores prepared at 25°C was 1.3-times higher than the spores prepared at 30°C. The sporulation temperature thus affects the resistance and germination properties of P. polymyxa spores. These results are useful for the production of improved bio-organic fertilizer.
多粘类芽孢杆菌 SQR-21 对尖孢镰刀菌具有拮抗作用,可用作生物防治剂,与有机物混合进行固态发酵后可生产生物有机肥。不同温度下制备的多粘类芽孢杆菌的芽孢,其特征在于二吡啶羧酸含量、耐热性、脂肪酸组成和发芽率。在 37°C 下制备的芽孢显示出比在 25°C 和 30°C 下制备的芽孢更高的耐热性。然而,发芽率与产孢温度呈负相关。在 25°C 下制备的芽孢的最大发芽率比在 30°C 下制备的芽孢高 1.3 倍。因此,产孢温度会影响多粘类芽孢杆菌芽孢的抗性和发芽特性。这些结果有助于生产改良的生物有机肥。