Jiangsu Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University, 210095, Nanjing, China.
Appl Microbiol Biotechnol. 2010 Jun;87(2):527-36. doi: 10.1007/s00253-010-2520-8. Epub 2010 Mar 23.
Bioorganic fertilizer containing Paenibacillus polymyxa SQR-21 showed very good antagonistic activity against Fusarium oxysporum. To optimize the role of P. polymyxa SQR-21 in bioorganic fertilizer, we conducted a study of spore germination under various conditions. In this study, L-asparagine, glucose, fructose and K(+) (AGFK), and sugars (glucose, fructose, sucrose, and lactose) plus L-alanine were evaluated to determine their ability to induce spore germination of two strains; P. polymyxa ACCC10252 and SQR-21. Spore germination was measured as a decrease in optical density at 600 nm. The effect of heat activation and germination temperature were important for germination of spores of both strains on AGFK in Tris-HCl. L-Alanine alone showed a slight increase in spore germination; however, fructose plus L-alanine significantly induced spore germination, and the maximum spore germination rate was observed with 10 mmol l(-1) L-alanine in the presence of 1 mmol l(-1) fructose in phosphate-buffered saline (PBS). In contrast, fructose plus L-alanine hardly induced spore germination in Tris-HCl; however, in addition of 10 mmol l(-1) NaCl into Tris-HCl, the percentages of OD(600) fall were increased by 19.6% and 24.3% for ACCC10252 and SQR-21, respectively. AGFK-induced spore germination was much more strict to germination temperature than that induced by fructose plus L-alanine. For both strains, fructose plus L-alanine-induced spore germination was not sensitive to pH. The results in this study can help to predict the effect of environmental factors and nutrients on spore germination diversity, which will be beneficial for bioorganic fertilizer storage and transportation to improve the P. polymyxa efficacy as biological control agent.
含解淀粉芽孢杆菌 SQR-21 的生物有机肥对尖孢镰刀菌表现出很好的拮抗活性。为了优化解淀粉芽孢杆菌 SQR-21 在生物有机肥中的作用，我们对其在不同条件下的孢子萌发进行了研究。在这项研究中，L-天冬酰胺、葡萄糖、果糖和 K(+)（AGFK）以及糖（葡萄糖、果糖、蔗糖和乳糖）加 L-丙氨酸被用来确定它们诱导两株菌（解淀粉芽孢杆菌 ACCC10252 和 SQR-21）孢子萌发的能力。通过测量 600nm 处的吸光度来检测孢子萌发。热激活和萌发温度对两株菌在 Tris-HCl 上利用 AGFK 进行孢子萌发的影响很重要。单独的 L-丙氨酸略微增加了孢子萌发；然而，果糖加 L-丙氨酸显著诱导了孢子萌发，在磷酸盐缓冲盐水（PBS）中存在 1mmol/L 果糖和 10mmol/L L-丙氨酸时，观察到最大的孢子萌发率。相比之下，果糖加 L-丙氨酸在 Tris-HCl 中几乎不能诱导孢子萌发；然而，在 Tris-HCl 中加入 10mmol/L NaCl 后，ACCC10252 和 SQR-21 的 OD(600)下降率分别增加了 19.6%和 24.3%。AGFK 诱导的孢子萌发对萌发温度的要求比果糖加 L-丙氨酸更严格。对于两株菌，果糖加 L-丙氨酸诱导的孢子萌发对 pH 不敏感。本研究的结果有助于预测环境因素和养分对孢子萌发多样性的影响，这将有助于生物有机肥的储存和运输，提高解淀粉芽孢杆菌作为生物防治剂的效果。
