Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Bioresour Technol. 2012 Mar;108:190-5. doi: 10.1016/j.biortech.2011.12.137. Epub 2012 Jan 4.
The effects of storage temperature (20, 30 and 40 °C), inoculum type (pure spores, an equal mix of spores and vegetative cells and pure vegetative cells) and water content (20%, 30% and 40%) on the survival and spore formation of the biocontrol agent, Paenibacillus polymyxa SQR-21, in a bioorganic fertilizer were modeled in a 3×3×3 factorial design. Bacterial and spore populations were monitored by plate count and fluorescence in situ hybridization (FISH). Temperature significantly affected survival of inoculants after storage for 60 days. Populations were 1.48 (plate counting) or 1.71 (FISH) times greater when stored at 20 °C compared to 40 °C. Inoculation of the fertilizer with pure spores led to the highest spore formation percentage (67.6% for plate counting, 94.2% for FISH). This study provides useful information for preservation of bioorganic fertilizer.
在 3×3×3 析因设计中，研究了储存温度（20、30 和 40°C）、接种物类型（纯孢子、孢子和营养细胞的等比例混合物以及纯营养细胞）和含水量（20%、30%和 40%）对生物有机肥中生防菌多粘类芽孢杆菌 SQR-21 的存活和孢子形成的影响。通过平板计数和荧光原位杂交（FISH）监测细菌和孢子数量。储存 60 天后，温度显著影响接种物的存活。与 40°C 相比，在 20°C 下储存时，细菌和孢子的数量分别增加了 1.48 倍（平板计数）或 1.71 倍（FISH）。用纯孢子接种肥料可导致最高的孢子形成百分比（平板计数为 67.6%，FISH 为 94.2%）。本研究为生物有机肥的保存提供了有用信息。
