非浸润性膀胱癌患者中 p16 和 DAPK 启动子基因区域的高甲基化。
Hypermethylation of p16 and DAPK promoter gene regions in patients with non-invasive urinary bladder cancer.
机构信息
1 Department of Urology, Medical University of Lodz, Poland.
出版信息
Arch Med Sci. 2011 Jun;7(3):512-6. doi: 10.5114/aoms.2011.23421. Epub 2011 Jul 11.
Abstract
INTRODUCTION
The aim of the study was to examine the frequency of methylation status in promoter regions of p16 and DAPK genes in patients with non-invasive bladder cancer.
MATERIAL AND METHODS
Forty-two patients (92.9% men, 73.8% smokers, 71.4% T1G1, 19.1% T1G2, 9.5% T1G3) and 36 healthy controls were studied. Isolation of genomic DNA from blood serum and methylation-specific PCR (MSP) were applied. Methylation status - methylated and unmethylated promoter regions of p16 and DAPK genes were analysed.
RESULTS
Seventeen out of 42 patients (40.5%) had the methylated p16 gene, while methylation of the DAPK gene was seen in 27 of 42 cases (64.3%). In 12 patients (28.6%) both analysed genes were methylated. A statistically significant (p = 0.046) higher frequency of DAPK gene methylation (71.4%) was observed in patients with lower grade (G1) bladder cancer.
CONCLUSIONS
Detection of the aberrant hypermethylation of DAPK and p16 genes in blood DNA from non-invasive bladder cancer patients might offer an effective means for earlier auxiliary diagnosis of the malignancy.
摘要
介绍
本研究旨在检测非浸润性膀胱癌患者 p16 和 DAPK 基因启动子区甲基化状态的频率。
材料与方法
研究纳入 42 名患者（92.9%为男性，73.8%为吸烟者，71.4%为 T1G1，19.1%为 T1G2，9.5%为 T1G3）和 36 名健康对照者。采用血清基因组 DNA 分离和甲基化特异性 PCR（MSP）检测。分析 p16 和 DAPK 基因启动子区甲基化状态 - 甲基化和非甲基化。
结果
42 名患者中有 17 名（40.5%）存在 p16 基因甲基化，42 例中有 27 例（64.3%）存在 DAPK 基因甲基化。在 12 例患者（28.6%）中，两个分析的基因均被甲基化。在低级别（G1）膀胱癌患者中，DAPK 基因甲基化频率显著更高（p = 0.046）。
结论
在非浸润性膀胱癌患者的血液 DNA 中检测到 DAPK 和 p16 基因的异常高甲基化，可能为恶性肿瘤的早期辅助诊断提供有效的手段。
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