Li Donghong, Gao Hua, Zang Jiatao, Diao Junlino, Liu Jiancang
State Key Laboratory of Trauma, Burn and Combined Injury, the 2nd Department of Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2011 Dec;28(6):1141-4.
A porous composite particle (CP) was fabricated by the methods of emulsification and cross-link based on chitosan, alginate and collagen protein, and the tranexamic acid-loaded composite particles (TACP) was prepared by immersing the composite particle into the solution of tranexamic acid and by freeze drying. In the hepatic and splenic hemorrhage model of rabbits, CP and TACP were randomly used as haemostatic agents, and the Suxiaozhixuefen (Flashclot) was used as control. The corresponding hemostatic time and bleeding amount were observed respectively. The hemostatic time of CP and Flashclot were (2.48 +/- 0.88) min and (3.07 +/- 0.84) min, respectively, no significant difference was observed. However, the hemostatic time of TACP was (1.90 +/- 0.75) min, which was significantly shorter than that of CP and Flashclot (P < 0.05). In the splenic bleeding model of rabbits, similar results were obtained with these three kinds of hemostatics. These results indicated that the CP based on chitosan, alginate and collagen protein displayed similar hemostatic efficiency to Flashclot. However, the TACP might be one of promising haemostatic powders due to its more excellent hemostatic efficiency.
基于壳聚糖、海藻酸盐和胶原蛋白，通过乳化和交联的方法制备了一种多孔复合颗粒（CP），并将复合颗粒浸入氨甲环酸溶液中，经冷冻干燥制备了载氨甲环酸复合颗粒（TACP）。在兔肝脾出血模型中，随机使用CP和TACP作为止血剂，并以速效止血粉作为对照。分别观察相应的止血时间和出血量。CP和速效止血粉的止血时间分别为（2.48±0.88）分钟和（3.07±0.84）分钟，未观察到显著差异。然而，TACP的止血时间为（1.90±0.75）分钟，明显短于CP和速效止血粉（P<0.05）。在兔脾出血模型中，这三种止血剂也得到了类似的结果。这些结果表明，基于壳聚糖、海藻酸盐和胶原蛋白的CP显示出与速效止血粉相似的止血效率。然而，TACP因其更优异的止血效率，可能是一种有前景的止血粉剂。
