Liu Xueyong, Deng Chunbo, Liu Jiquan, Li Jianjun, Sui Guoxin
Department of Orthopedic Surgery, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2011 Dec;28(6):1159-64.
The present research was to study the biocompatibility of a composite of hydroxyapatite (HA), carbon fiber (CF) and polyetheretherket-one (PEEK) by co-culturing with the osteoblasts in vitro. Cell relative growth (RGR) was used as a quantitative assessment for cytotoxicity of the biomaterials by CCK-8. The proliferation index of the co-cultured cells and ALP activity was measured to study the effect of PEEK-HA-CF composites. Morphological properties of the osteoblast cells in vitro were observed by scanning electro-microscopy (SEM). The PEEK-HA-CF materials have no cytotoxicity to osteoblasts. The proliferation index of PEEK-HA-CF was higher than that of Ti alloy group, but these was no significant difference compared to that of control group. The ALP activity was the highest on PEEK-HA-CF composites surface after 7 days. The osteoblast cells co-cultured with the PEEK-HA-CF composite were adhered well to the biomaterial as observed under the SEM. The results suggested that the PEEK-HA-CF composites had good biocompatibility in vitro and might be a novel orthopedic implanted material.
本研究旨在通过与成骨细胞体外共培养，研究羟基磷灰石（HA）、碳纤维（CF）和聚醚醚酮（PEEK）复合材料的生物相容性。采用细胞相对生长率（RGR）通过CCK-8对生物材料的细胞毒性进行定量评估。测量共培养细胞的增殖指数和碱性磷酸酶（ALP）活性，以研究PEEK-HA-CF复合材料的作用。通过扫描电子显微镜（SEM）观察体外成骨细胞的形态学特性。PEEK-HA-CF材料对成骨细胞无细胞毒性。PEEK-HA-CF的增殖指数高于钛合金组，但与对照组相比无显著差异。7天后，PEEK-HA-CF复合材料表面的ALP活性最高。在SEM下观察到，与PEEK-HA-CF复合材料共培养的成骨细胞与生物材料良好黏附。结果表明，PEEK-HA-CF复合材料在体外具有良好的生物相容性，可能是一种新型的骨科植入材料。
