Zou Wei, Liu Wu-jiang
Department of Surgery, The Hospital of Chongqing University of Post and Telecommunication, Chongqing 400065, China.
Zhonghua Nan Ke Xue. 2012 Jan;18(1):63-6.
To detect the mRNA and protein expressions of MIP-1alpha in EPS and determine their significance in the sub-typing of chronic prostatitis.
We collected samples of expressed prostatic secretion (EPS) from 50 cases of chronic prostatitis, including 16 cases of chronic bacterial prostatitis (CBP), 23 cases of chronic nonbacterial prostatitis/chronic pelvic pain syndrome (CPPS) (11 CPPS IIIA, 12 CPPS IIIB), and 11 cases of type-IV asymptomatic inflammatory prostatitis (AIP). Another 15 healthy volunteers were included as normal controls. The mRNA and protein levels of MIP-1alpha in EPS were determined by RT-PCR and ELISA, respectively, followed by statistical analysis with SPSS 15.0.
The mRNA expression of MIP-1alpha was markedly higher in the CPPS IIIA and CPPS IIIB groups than in the others (P<0.05). The protein level of MIP-1alpha was (1174.3 +/- 89.2) pg/ml in CPPS IIIA and (842.3 +/- 76.2) pg/ml in CPPS IIIB, significantly higher than (198.0 +/- 37.8) pg/ml in the control, (347.0 +/- 61.6) pg/ml in CBP and (292.0 +/- 56.4) pg/ml in type-IV AIP (P<0.05).
Determination of mRNA and protein levels of MIP-1alpha in EPS may help the sub-typing and diagnosis of chronic prostatitis.
检测前列腺按摩液（EPS）中巨噬细胞炎性蛋白-1α（MIP-1α）的mRNA和蛋白表达，并探讨其在慢性前列腺炎分型中的意义。
收集50例慢性前列腺炎患者的EPS样本，其中慢性细菌性前列腺炎（CBP）16例，慢性非细菌性前列腺炎/慢性盆腔疼痛综合征（CPPS）23例（ⅢA型11例，ⅢB型12例），Ⅳ型无症状性炎性前列腺炎（AIP）11例。另选取15名健康志愿者作为正常对照。分别采用逆转录-聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）法检测EPS中MIP-1α的mRNA和蛋白水平，然后用SPSS 15.0软件进行统计学分析。
CPPSⅢA型和ⅢB型组中MIP-1α的mRNA表达明显高于其他组（P<0.05）。CPPSⅢA型中MIP-1α的蛋白水平为（1174.3±89.2）pg/ml，CPPSⅢB型中为（842.3±76.2）pg/ml，显著高于对照组的（198.0±37.8）pg/ml、CBP组的（347.0±61.6）pg/ml和Ⅳ型AIP组的（292.0±56.4）pg/ml（P<0.05）。
检测EPS中MIP-1α的mRNA和蛋白水平可能有助于慢性前列腺炎的分型和诊断。
