Hochreiter W W, Nadler R B, Koch A E, Campbell P L, Ludwig M, Weidner W, Schaeffer A J
Departments of Urology, Northwestern University Medical School, Chicago, Illinois, USA.
Urology. 2000 Dec 20;56(6):1025-9. doi: 10.1016/s0090-4295(00)00844-x.
Chronic prostatitis/chronic pelvic pain syndrome (CPPS) is a disorder characterized by pelvic pain and varying degrees of inflammation exhibited in expressed prostatic secretions (EPS). To provide objective parameters of inflammation, we measured the cytokines interleukin 8 (IL-8) and epithelial neutrophil activating peptide 78 (ENA-78) in EPS of healthy men, men with benign prostatic hyperplasia (BPH), men with bacterial prostatitis (BP), and men with chronic prostatitis/CPPS.
Enzyme-linked immunosorbent assays of the EPS for IL-8 and ENA-78 were done in 63 men: control (n = 9), BPH (n = 6), BP (n = 3), inflammatory CPPS (National Institutes of Health [NIH] category IIIa) (n = 17), noninflammatory CPPS (NIH category IIIb) (n = 17), and asymptomatic inflammatory prostatitis (NIH category IV) (n = 11).
IL-8 was detectable in all patients, and ENA-78 was detectable in all except 2 patients (threshold of detection 10 pg/mL for IL-8, 15 pg/mL for ENA-78). Mean levels of IL-8 [ENA-78] were similar in control (3010 pg/mL [423 pg/mL]), BPH (3341 pg/mL [98 pg/mL]), and IIIb (2751 pg/mL [335 pg/mL]) groups. Both cytokine levels were higher in BP (11,175 pg/mL [13,761 pg/mL]), IIIa (10,418 pg/mL [2240 pg/mL]), and IV (8571 pg/mL [1865 pg/mL]) groups. A statistically significant difference between the control group versus BP, IIIa, and IV (P <0.05) groups was found for IL-8 but not for ENA-78.
IL-8 and ENA-78 are frequently elevated in the EPS of men with BP, CPPS IIIa, and asymptomatic inflammatory prostatitis category IV. These cytokines are direct mediators of leukocyte accumulation and activation at inflammatory sites and may be responsible, in part, for the presence of inflammatory reaction in the prostate.
慢性前列腺炎/慢性盆腔疼痛综合征（CPPS）是一种以盆腔疼痛和前列腺液（EPS）中表现出不同程度炎症为特征的疾病。为了提供炎症的客观参数，我们检测了健康男性、良性前列腺增生（BPH）男性、细菌性前列腺炎（BP）男性以及慢性前列腺炎/CPPS男性的EPS中的细胞因子白细胞介素8（IL-8）和上皮中性粒细胞激活肽78（ENA-78）。
对63名男性的EPS进行IL-8和ENA-78的酶联免疫吸附测定：对照组（n = 9）、BPH组（n = 6）、BP组（n = 3）、炎症性CPPS（美国国立卫生研究院 [NIH] IIIa类）组（n = 17）、非炎症性CPPS（NIH IIIb类）组（n = 17）以及无症状性炎症性前列腺炎（NIH IV类）组（n = 11）。
所有患者的EPS中均可检测到IL-8，除2例患者外所有患者的EPS中均可检测到ENA-78（IL-8的检测阈值为10 pg/mL，ENA-78的检测阈值为15 pg/mL）。对照组（3010 pg/mL [423 pg/mL]）、BPH组（3341 pg/mL [98 pg/mL]）和IIIb组（2751 pg/mL [335 pg/mL]）的IL-8 [ENA-78]平均水平相似。BP组（11,175 pg/mL [13,761 pg/mL]）、IIIa组（10,418 pg/mL [2240 pg/mL]）和IV组（8571 pg/mL [1865 pg/mL]）的两种细胞因子水平均较高。IL-8在对照组与BP组、IIIa组和IV组（P <0.05）之间存在统计学显著差异，而ENA-78则无。
IL-8和ENA-78在BP、CPPS IIIa和无症状性炎症性前列腺炎IV类男性的EPS中经常升高。这些细胞因子是炎症部位白细胞聚集和激活的直接介质，可能部分导致前列腺炎症反应的存在。
