University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University, Chandigarh 160014, India.
Neurogastroenterol Motil. 2012 Apr;24(4):366-e170. doi: 10.1111/j.1365-2982.2011.01861.x. Epub 2012 Feb 1.
In recent years the interface between neuropsychiatry and gastroenterology has converged in to a new discipline referred to as enteric neuroscience. Implications of brain-gut communication in the pathogenesis of psychiatric disorders indicate a possible role of suitably packaged/delivered probiotics as newer therapeutic options. In the present study probable role of per-oral administration of free Lactobacillus acidophilus (LAB) and LAB loaded alginate beads in attenuation of the symptoms associated with chronic fatigue syndrome (CFS) were evaluated.
Chronic fatigue syndrome following physical fatigue was induced in rats by forcing them to swim (forced swim test; FST) in water till exhaustion, after weighing them down with 10% their body weight, daily for 28 days. Immobility (I) and postswim fatigue time (PSF) were taken as suitable markers. Free LAB and LAB loaded floating beads (FBs) were administered, from 21 to 28 days.
Immobility and PSF were found to increase considerably in FST rats (665 ± 22 s and 196 ± 6 s) as compared with the naïve (32 ± 7 s and 22 ± 2 s) at 20 days, establishing severe fatigue like behavior. FST control group exhibited significant (P < 0.05) hypertrophy of spleen, hypotrophy of thymus, and increased oxido-nitrosative stress in brain and tumor necrosis factor-α (TNF-α) levels in serum. Treatment with LAB and LAB FBs significantly decreased I and PSF and attenuated (P < 0.05) oxido-nitrosative stress and TNF-α levels. Spleen and thymus were also restored to their original size in this group.
CONCLUSIONS & INFERENCES: The findings suggest a valuable therapeutic role of LAB especially when incorporated into alginate beads for the treatment of CFS.
近年来，神经精神病学和胃肠病学之间的界面汇聚成一个新的学科，称为肠神经科学。大脑-肠道通讯在精神障碍发病机制中的作用表明，适当包装/递送的益生菌可能作为新的治疗选择发挥作用。在本研究中，评估了口服给予游离嗜酸乳杆菌（LAB）和载有 LAB 的藻酸盐珠在减轻慢性疲劳综合征（CFS）相关症状中的可能作用。
通过在水中强迫大鼠游泳（强迫游泳试验；FST）至筋疲力尽，将它们的体重减轻 10%，每天 28 天，在大鼠中诱导体力疲劳后的慢性疲劳综合征。不动性（I）和游泳后疲劳时间（PSF）被用作合适的标志物。从第 21 天到第 28 天，给予游离 LAB 和载有 LAB 的漂浮珠（FB）。
与未处理的大鼠（32 ± 7 s 和 22 ± 2 s）相比，FST 大鼠的 FST 中的不动性和 PSF 显著增加（665 ± 22 s 和 196 ± 6 s）在第 20 天，表现出严重的疲劳样行为。FST 对照组表现出显著的（P < 0.05）脾脏肿大、胸腺萎缩以及大脑中的氧化应激和一氧化氮应激增加和血清肿瘤坏死因子-α（TNF-α）水平升高。用 LAB 和 LAB FB 治疗可显著降低 I 和 PSF，并减轻（P < 0.05）氧化应激和 TNF-α水平。在该组中，脾脏和胸腺也恢复到原来的大小。
这些发现表明 LAB 具有有价值的治疗作用，特别是当将其纳入藻酸盐珠中用于治疗 CFS 时。
