Pharmacology Division, University Institute of Pharmaceutical Sciences, UGC Center of Advanced Study, Panjab University, Chandigarh 160014, India.
J Neuroimmunol. 2010 Sep 14;226(1-2):3-7. doi: 10.1016/j.jneuroim.2010.05.021. Epub 2010 May 26.
Various putative theories involved in the development of chronic fatigue syndrome revolve around the role of stress, infection and oxidative stress. Scientific evidence highlighting the protective role of nutritional supplements in chronic fatigue syndrome is lacking. Based on these assumptions, the present study was designed to evaluate the effect of olive extract in a mouse model of immunologically-induced fatigue, wherein purified lipopolysaccharide (LPS) and Brucella abortus (BA) antigen were used as immunogens. The assessment of chronic fatigue syndrome was based on immobility period during chronic water-immersion stress test for 10 min daily. The stress-induced hyperalgesia was measured by tail withdrawal latency. Mice challenged with LPS or BA for 19 days showed significant increase in the immobility time, hyperalgesia and oxidative stress on the 19th day. Serum tumor necrosis factor-alpha (TNF-α) levels were also markedly increased with LPS or BA challenge. Concurrent treatment with olive extract resulted in a significant decrease in the immobility time as well as hyperalgesia. There was significant attenuation of oxidative stress as well as serum TNF-α levels. The results of the present study strongly indicate the role of oxidative stress and immunological activation in the pathophysiology of chronic fatigue syndrome and highlight the valuable role of olive extract in combating chronic fatigue syndrome.
各种与慢性疲劳综合征发展相关的假说理论都围绕着压力、感染和氧化应激的作用展开。缺乏突出营养补充剂在慢性疲劳综合征中具有保护作用的科学证据。基于这些假设，本研究旨在评估橄榄油提取物对免疫诱导性疲劳小鼠模型的影响，其中使用纯化脂多糖（LPS）和布鲁氏菌流产抗原作为免疫原。慢性疲劳综合征的评估基于每天 10 分钟慢性浸水应激试验中的不动期。通过尾巴退缩潜伏期测量应激诱导的痛觉过敏。用 LPS 或 BA 挑战 19 天的小鼠在第 19 天表现出明显的不动时间增加、痛觉过敏和氧化应激。用 LPS 或 BA 挑战后，血清肿瘤坏死因子-α（TNF-α）水平也明显升高。与橄榄油提取物同时治疗可显著减少不动时间和痛觉过敏。氧化应激和血清 TNF-α水平明显减弱。本研究的结果强烈表明氧化应激和免疫激活在慢性疲劳综合征的病理生理学中的作用，并突出了橄榄油提取物在对抗慢性疲劳综合征方面的有价值作用。
