Department of Pharmaceutical Sciences, Vaccine Nanotechnology Laboratory, College of Pharmacy and Health Sciences, Mercer University, Atlanta, GA 30341, USA.
J Drug Target. 2012 May;20(4):338-46. doi: 10.3109/1061186X.2011.654122. Epub 2012 Feb 1.
Various approaches have been evaluated for generation of efficient immune response against tumor antigens. Our approach exploits usage of particulate delivery to generate immune response against prostate cancer antigens.
The aim of this study was to evaluate the efficacy of prostate cancer vaccine derived from a murine prostate cancer cell line, TRAMP C2 in murine model via oral route using aleuria aurantia lectin as a targeting ligand for M-cells in the intestinal Peyer's patches.
The whole cell lysate (WCL) was obtained from TRAMP C2 murine prostate cancer cell line and was formulated into particles using one step spray drying process. For in vivo studies, 4-6 week old C57BL/6 male mice were vaccinated orally biweekly for 10 weeks. Serum samples were analyzed at regular intervals to determine serum IgG levels. The mice were then challenged with live TRAMP C2 cells to determine efficacy of the vaccine.
The serum IgG levels of vaccinated animals were higher compared to that of the controls. Moreover, the tumor growth was retarded significantly in the vaccinated mice compared to that of controls (p < 0.001).
The above findings suggest that oral particulate WCL vaccine can trigger an immune response against prostate cancer antigens.
已经评估了各种方法来产生针对肿瘤抗原的有效免疫反应。我们的方法利用颗粒递送来产生针对前列腺癌抗原的免疫反应。
本研究旨在通过口服途径使用美洲商陆凝集素作为肠道派尔集合淋巴结中 M 细胞的靶向配体，评估源自鼠前列腺癌细胞系 TRAMP C2 的前列腺癌疫苗在鼠模型中的疗效。
从 TRAMP C2 鼠前列腺癌细胞系获得全细胞裂解物 (WCL)，并使用一步喷雾干燥工艺将其制成颗粒。对于体内研究，4-6 周龄 C57BL/6 雄性小鼠每隔两周口服接种 10 周。定期分析血清样本以确定血清 IgG 水平。然后用活的 TRAMP C2 细胞对小鼠进行攻毒，以确定疫苗的疗效。
与对照组相比，接种动物的血清 IgG 水平更高。此外，与对照组相比，接种疫苗的小鼠的肿瘤生长明显延迟 (p < 0.001)。
上述发现表明口服颗粒 WCL 疫苗可以引发针对前列腺癌抗原的免疫反应。
