与人类肝细胞癌进展及预后相关的 runt 相关转录因子 3 表达缺失。
Loss of runt-related transcription factor 3 expression associated with human hepatocellular carcinoma progression and prognosis.
作者信息
Li Jianguo, Jiang Xiaojie
机构信息
Zhangzhou Affiliated Hospital, Fujian Medical University, Zhangzhou, China.
出版信息
Asian Pac J Cancer Prev. 2011;12(9):2285-90.
BACKGROUND
This study aimed to evaluate the expression level of runt-related transcription factor 3 (RUNX3) in human primary hepatocellular carcinomas (HCCs) and its relationship with the clinic pathological features.
METHODS
RUNX3 expression was analyzed by real-time fluorescent quantitative PCR, immunohistochemistry and Western blotting in HCC cells and tissues.
RESULTS
RUNX3 RNA and protein expression was decreased in HCC tissues compared with the adjacent normal tissues (P< 0.001), mRNA frequently being down-regulated in HCC cell lines (66.67%, 4/6). Low expression of RUNX3 showed a significant correlation with cirrhosis (P = 0.028), histologic type (P = 0.000) and lymph node metastasis (P= 0.004).
CONCLUSION
RUNX3 expression is deleted or decreased in HCCs and cell lines, in association with progression and prognosis.
背景
本研究旨在评估人类原发性肝细胞癌（HCC）中 runt 相关转录因子 3（RUNX3）的表达水平及其与临床病理特征的关系。
方法
采用实时荧光定量 PCR、免疫组织化学和蛋白质印迹法分析 HCC 细胞和组织中 RUNX3 的表达情况。
结果
与相邻正常组织相比，HCC 组织中 RUNX3 的 RNA 和蛋白质表达降低（P<0.001），在 HCC 细胞系中 mRNA 也经常下调（66.67%，4/6）。RUNX3 的低表达与肝硬化（P = 0.028）、组织学类型（P = 0.000）和淋巴结转移（P = 0.004）显著相关。
结论
HCC 及其细胞系中 RUNX3 的表达缺失或降低，与病情进展和预后相关。
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