Paulus W, Roggendorf W
Institute of Brain Research, University of Tübingen, Federal Republic of Germany.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;56(1):67-70. doi: 10.1007/BF02890003.
Normal, reactive, and neoplastic astrocytes express two types of intermediate filament (IF) proteins, namely glial fibrillary acidic protein (GFAP) and vimentin. Their submicroscopical distribution in vivo is so far unknown. We therefore investigated four malignant gliomas by electron microscopy, applying postembedding double immunogold labeling. The IF proteins were randomly scattered over the same filament bundles, as in previous experiments on glioma cultures. No clustering or preferential intracytoplasmic location of either IF protein was visible. The demonstration of IF proteins within nuclei gives some support to the suggested intranuclear functions of IF proteins.
正常、反应性和肿瘤性星形胶质细胞表达两种中间丝（IF）蛋白，即胶质纤维酸性蛋白（GFAP）和波形蛋白。它们在体内的亚显微镜分布迄今尚不清楚。因此，我们通过电子显微镜研究了4例恶性胶质瘤，采用包埋后双重免疫金标记法。与先前对胶质瘤培养物的实验一样，IF蛋白随机分散在相同的丝束上。未观察到任何一种IF蛋白的聚集或优先胞浆内定位。IF蛋白在细胞核内的显示为IF蛋白的核内功能假说提供了一些支持。
