Shimbo Y, Yamazaki K, Ikuta F
Department of Pathology, Niigata University, Japan.
Acta Neuropathol. 1987;74(1):1-8. doi: 10.1007/BF00688331.
Glial filaments contain vimentin and glial fibrillary acidic protein (GFAP). The question of how glial filaments change morphologically according to the expression of vimentin and/or GFAP has remained unclear. In this study, immunohistochemical and ultrastructural examinations were performed on the subcutaneously transplanted tumors of two clones (F6B3 and G10A10) derived from a mouse glioma. F6B3 tumor expressed GFAP and vimentin in large quantities. G10A10 tumor expressed plenty of vimentin but only a little of GFAP. Ultrastructurally, F6B3 tumor contained abundant cytoprocesses in most of which numerous intermediate filaments (IFs) were arranged in a parallel array. On the other hand, only a small number of the processes were seen in G10A10 tumor, which showed a few IFs arranged either randomly or sparsely in the processes. Both tumors commonly had the IFs accompanied by visible sidearms, but there was a difference in that the smooth and firm IFs were confined to part of F6B3 tumor. Thus, the comparison made between the two models presented differences in the content, arrangement and morphology of IFs, as well as in the manner of GFAP expression, suggesting correlation between these differences.
神经胶质丝包含波形蛋白和神经胶质纤维酸性蛋白（GFAP）。神经胶质丝如何根据波形蛋白和/或GFAP的表达在形态上发生变化的问题仍不清楚。在本研究中，对源自小鼠胶质瘤的两个克隆（F6B3和G10A10）的皮下移植肿瘤进行了免疫组织化学和超微结构检查。F6B3肿瘤大量表达GFAP和波形蛋白。G10A10肿瘤表达大量波形蛋白但仅少量GFAP。在超微结构上，F6B3肿瘤含有丰富的细胞突起，其中大多数有大量中间丝（IFs）呈平行排列。另一方面，在G10A10肿瘤中仅可见少数突起，其中有一些IFs在突起中随机或稀疏排列。两种肿瘤中的IFs通常都伴有可见的侧臂，但不同之处在于，光滑且紧密的IFs局限于F6B3肿瘤的一部分。因此，对这两种模型的比较显示出IFs在含量、排列和形态以及GFAP表达方式上存在差异，表明这些差异之间存在相关性。
