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弱碳水化合物-碳水化合物相互作用介导的细胞黏附模型系统。

Model system for cell adhesion mediated by weak carbohydrate-carbohydrate interactions.

机构信息

Institute of Physical Chemistry, University of Göttingen, Tammannstrasse 6, 37077 Göttingen, Germany.

出版信息

J Am Chem Soc. 2012 Feb 22;134(7):3326-9. doi: 10.1021/ja210304j. Epub 2012 Feb 10.

Abstract

The multivalent carbohydrate-carbohydrate interaction between membrane-anchored epitopes derived from the marine sponge Microciona prolifera has been explored by colloidal probe microscopy. An in situ coupling of sulfated and non-sulfated disaccharides to membrane-coated surfaces was employed to mimic native cell-cell contacts.The dynamic strength of the homomeric self-association was measured as a function of calcium ions and loading rate. A deterministic model was used to estimate the basic energy landscape and number of participating bonds in the contact zone.

摘要

通过胶体探针显微镜研究了来源于海洋海绵 Microciona prolifera 的膜锚定表位的多价碳水化合物-碳水化合物相互作用。通过将磺酸化和非磺酸化二糖原位偶联到膜包被表面来模拟天然细胞-细胞接触。作为钙离子和加载速率的函数来测量同型自组装的动态强度。使用确定性模型来估计接触区域中的基本能量景观和参与键的数量。

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