Sun Yung-Shin, Zhu X D
Department of Physics, Fu-Jen Catholic University, New Taipei City, Taiwan.
Department of Physics, University of California at Davis, Davis, CA, USA.
Instrum Sci Technol. 2017;45(5):506-524. doi: 10.1080/10739149.2016.1278017. Epub 2017 Feb 13.
Carbohydrates present on cell surfaces mediate cell behavior through interactions with other biomolecules. Due to their structural complexity, diversity, and heterogeneity, it is difficult to fully characterize a variety of carbohydrates and their binding partners. As a result, novel technologies for glycomics applications have been developed, including carbohydrate microarrays and label-free detection methods. In this paper, we report using the combination of oligosaccharide microarrays and the label-free oblique-incidence reflectivity difference (OI-RD) microscopy for real-time characterization of oligosaccharide binding proteins. Aminated human milk oligosaccharides were immobilized on epoxy-coated glass substrates as microarrays for reactions with Family 1 of solute binding proteins from subsp. (). Binding affinities of these protein-oligosaccharide interactions showed preferences of Family 1 of solute binding proteins to host glycans, which helps in characterizing the complex process of human milk oligosaccharides foraging by
细胞表面的碳水化合物通过与其他生物分子的相互作用介导细胞行为。由于其结构的复杂性、多样性和异质性，很难完全表征各种碳水化合物及其结合伙伴。因此，已经开发了用于糖组学应用的新技术，包括碳水化合物微阵列和无标记检测方法。在本文中，我们报告了使用寡糖微阵列和无标记斜入射反射率差（OI-RD）显微镜相结合的方法来实时表征寡糖结合蛋白。将胺化人乳寡糖固定在环氧涂层玻璃基板上作为微阵列，用于与来自亚种的溶质结合蛋白家族1进行反应。这些蛋白质-寡糖相互作用的结合亲和力显示出溶质结合蛋白家族1对宿主聚糖的偏好，这有助于表征人乳寡糖觅食的复杂过程。
