Uttah Emmanuel, Ibeh Dominic C
Department of Biological Sciences, Faculty of Science, Cross River University of Technology, Calabar, Nigeria.
J Vector Borne Dis. 2011 Dec;48(4):197-204.
BACKGROUND & OBJECTIVES: The study was aimed at determining the pattern of co-occurrence of species of microfilaraemia between onchocerciasis endemic and sporadic populations.
From every consenting person of one year and above, 50 μl of day and night blood samples were collected and processed respectively with Haemotoxylin and Giemsa as vital stains. Two skin snips (one each from the waist and the shoulder) were also taken from these individuals and processed.
Results showed single species microfilaraemia (86.4 and 82.3%), double species microfilaraemia (12.2 and 16.9%) and triple species microfilaraemia (1.4 and 0.7%) for endemic and sporadic populations respectively. All the species had single species microfilaraemia mostly, but Mansonella perstans and Loa loa showed greatest tendency towards double and triple species microfilaraemia. The prevalence of Wuchereria bancrofti microfilaraemia among those positive for Onchocerca volvulus was significantly lower than the overall prevalence of Wuchereria bancrofti. Wuchereria bancrofti microfilaraemia was most common among those who had L. loa microfilaraemia. Wuchereria bancrofti microfilarial intensity was higher among those with M. perstans microfilaraemia than among those positive for any of the other filarial species. Similarly, the intensity of M. perstans microfilaraemia among those positive for W. bancrofti exceeded the overall intensity of M. perstans.
It is concluded that there was no definite pattern in mf densities discernible from co- occurrence infections either in the onchocerciasis endemic or sporadic population. There could be varied outcomes of onchocerciasis infection attributable to positive or negative regulatory effects of other pathogens harbored by the victims.
本研究旨在确定盘尾丝虫病流行区和散发区人群中微丝蚴血症的共现模式。
从每位年满一岁及以上且同意参与的人身上分别采集50μl白天和夜晚的血样，并用苏木精和吉姆萨作为活体染色剂进行处理。还从这些个体身上取了两片皮肤组织（一片来自腰部，一片来自肩部）并进行处理。
结果显示，流行区和散发区人群中分别有单物种微丝蚴血症（86.4%和82.3%）、双物种微丝蚴血症（12.2%和16.9%）以及三物种微丝蚴血症（1.4%和0.7%）。所有物种大多为单物种微丝蚴血症，但常现曼森线虫和罗阿丝虫出现双物种和三物种微丝蚴血症的倾向最大。在盘尾丝虫阳性者中，班氏吴策线虫微丝蚴血症的患病率显著低于班氏吴策线虫的总体患病率。班氏吴策线虫微丝蚴血症在有罗阿丝虫微丝蚴血症的人群中最为常见。有常现曼森线虫微丝蚴血症者中班氏吴策线虫微丝蚴的强度高于其他任何丝虫物种阳性者。同样，在班氏吴策线虫阳性者中，常现曼森线虫微丝蚴血症的强度超过了常现曼森线虫的总体强度。
得出的结论是，在盘尾丝虫病流行区或散发区人群中，从共感染情况来看，微丝蚴密度没有明确的模式。盘尾丝虫病感染可能因受害者体内携带的其他病原体的正负调节作用而产生不同结果。
