采用不同方法评估新型止血剂 Ankaferd Blood Stopper®的细胞毒性。
Evaluation of cytotoxicity of a new hemostatic agent Ankaferd Blood Stopper® using different assays.
机构信息
Bezmialem Vakif University, Istanbul, Turkey.
出版信息
Hum Exp Toxicol. 2012 Aug;31(8):780-7. doi: 10.1177/0960327111434949. Epub 2012 Feb 1.
The aim of this study is to investigate the effects of the Ankaferd Blood Stopper® (ABS), on cell viability, cytotoxicity, and erythrocyte numbers in in vitro cultured human blood cells. We studied the cytotoxic effects of the ABS using lactate dehydrogenase (LDH) assay, cell proliferation (WST-1) assay and hemolytic assay. The cytotoxicity increased when cells were treated with ABS dilutions of 5%, 12.5%, 25%, and 50% (p < 0.05). Moreover, treatment of the cells with the same concentrations significantly elevated the cell number at 24 and 48 h (p < 0.05). ABS causes a significant increase (p < 0.05) in the hemolytic activity on human erythrocytes and hemolytic activity increases with increase in ABS concentrations. The red blood cell aggregation and cell membrane disruption during the coagulation process lead to induction of hemolytic activity and increase of LDH level in cell culture medium. In addition, ABS has proliferative effects on human leukocytes. Based on these results, ABS can be used as an alternative blood stopping agent safely.
本研究旨在探讨安可血®（ABS）对体外培养的人血细胞的细胞活力、细胞毒性和红细胞数量的影响。我们使用乳酸脱氢酶（LDH）测定法、细胞增殖（WST-1）测定法和溶血测定法研究了 ABS 的细胞毒性作用。当用 5%、12.5%、25%和 50%的 ABS 稀释液处理细胞时，细胞毒性增加（p<0.05）。此外，相同浓度的 ABS 处理可显著增加细胞在 24 和 48 小时的数量（p<0.05）。ABS 可显著增加（p<0.05）对人红细胞的溶血活性，且溶血活性随 ABS 浓度的增加而增加。在凝血过程中，红细胞聚集和细胞膜破裂导致溶血活性的诱导和细胞培养液中 LDH 水平的升高。此外，ABS 对人白细胞具有增殖作用。基于这些结果，ABS 可作为一种安全的替代止血剂使用。
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