Department of Pathophysiology, University of Belgrade, Serbia.
Hum Exp Toxicol. 2012 Apr;31(4):387-96. doi: 10.1177/0960327111433899. Epub 2012 Feb 1.
The aim of our study was to evaluate the effects of cold stress on hepatic oxidative damage during binge drinking in rats. Male Wistar rats were divided into the following groups: group 1: control; group 2: ethanol-treated; group 3: stress-exposed; group 4: stress-exposed and ethanol-treated group. Oxidative and nitrosative stress parameters in the liver were determined spectrophotometrically, 12 h after treatment. Liver malondialdehyde concentration was significantly higher in group 4 when compared with groups 2 and 3. The highest increase in nitric oxide concentration was demonstrated in group 4 in comparison with groups 2 and 3. Superoxide dismutase (SOD) activity was significantly lower in group 4 when compared with groups 2 and 3. Ethanol administration induced a larger decrease in the activity of copper-/zinc-SOD in group 4 in comparison with group 2. Activity of manganese-SOD (Mn-SOD) was significantly higher in groups 3 and 4, when compared with control values, but the greatest increase in the activity of Mn-SOD was demonstrated in group 2. We also evaluated statistically significant decrease in the level of reduced gluthatione in the liver of group 4 in comparison with group 3. Based on our study, it can be concluded that cold-exposed stress and binge ethanol drinking have additive effects in imbalance between pro-oxidant and antioxidant defense system in liver.
我们的研究目的是评估在大鼠 binge 饮酒期间冷应激对肝氧化损伤的影响。雄性 Wistar 大鼠分为以下几组：第 1 组：对照组；第 2 组：乙醇处理组；第 3 组：应激暴露组；第 4 组：应激暴露和乙醇处理组。治疗 12 小时后，通过分光光度法测定肝脏的氧化和硝化应激参数。与第 2 组和第 3 组相比，第 4 组的肝丙二醛浓度明显升高。与第 2 组和第 3 组相比，第 4 组的一氧化氮浓度增加最多。与第 2 组和第 3 组相比，第 4 组的超氧化物歧化酶 (SOD) 活性明显降低。与第 2 组相比，第 4 组的铜/锌-SOD 活性下降更大。与对照组相比，第 3 组和第 4 组的锰-SOD (Mn-SOD) 活性显著升高，但 Mn-SOD 活性的最大增加发生在第 2 组。我们还评估了与第 3 组相比，第 4 组的肝脏还原型谷胱甘肽水平显著下降。基于我们的研究，可以得出结论，冷暴露应激和 binge 乙醇摄入在肝脏的促氧化剂和抗氧化防御系统之间的平衡中具有相加作用。
