Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Strasse 9, 60438 Frankfurt, Germany.
Chembiochem. 2012 Mar 5;13(4):534-7. doi: 10.1002/cbic.201100721. Epub 2012 Feb 1.
A major limitation of solution NMR is molecular tumbling, which is often too slow for detection. Here we demonstrate that solid-state NMR spectroscopy in combination with flash freezing of cells can be used to detect proteins in the cellular environment and provides information on backbone chemical shifts.
溶液 NMR 的一个主要限制是分子转动，其通常太慢而无法被检测到。在这里，我们证明了固态 NMR 光谱学与细胞的快速冷冻相结合，可用于检测细胞环境中的蛋白质，并提供有关骨架化学位移的信息。
