Metabolic heat production during repeated cold stress in adult and aged male C57BL/6J mice.

Adult and aged male C57BL/6J mice were subjected to three consecutive 3-hour cold stress tests (partial physical restraint at 6 degrees C), repeated 2 weeks apart. Body mass and colonic temperature were measured before each test, and colonic temperature, O2 consumption, and CO2 production were measured during cold exposure. The slopes of colonic temperature and heat production and the mean metabolic heat production were calculated for each animal. Adult mice showed stronger cold tolerance compared to aged mice and also exhibited habituation to cold exposure (improvement of cold tolerance with repeated tests). Mean metabolic heat production during cold exposure was greater in adult mice, and only adults demonstrated significant increases across tests in both metabolic heat production and slope of metabolic heat production over time. We hypothesize that reduced cold tolerance in aged mice is related mainly to a decrease in metabolic heat production. Increased metabolic heat production in subsequent tests in adults is a probable mechanism for habituation to repeated cold exposure.