Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
J Microencapsul. 2012;29(5):437-44. doi: 10.3109/02652048.2012.655333. Epub 2012 Feb 2.
The purpose of this study was to develop a sonosensitizer-loaded multi-functional ultrasound (US) contrast agent for both tumour therapy and imaging. The hematoporphyrin (HP)-encapsulated poly(lactic-co-glycolic acid) microbubbles (HP-PLGA-MBs) were prepared and filled with perfluorocarbon gases. The enhancement of US imaging and its sonodynamically induced anti-tumour effect were evaluated by both in vitro and in vivo experiments. The HP-PLGA-MBs have a narrow size distribution and smooth surface with a mean diameter of 702.6 ± 56.8 nm and HP encapsulation efficiency of 63.50 ± 1.26% and drug-loading efficiency of 2.15 ± 0.13%. The HP-PLGA-MBs could well enhance the ultrasound imaging both in vitro and in vivo. A significant anti-tumour effect was obtained by HP-PLGA-MBs mediated sonodynamic therapy. The tumour growth rate and the tumour proliferation index were the lowest in the HP-PLGA-MBs plus sonication group. And the tumour cell apoptotic index was the biggest in the HP-PLGA-MBs plus sonication group. In conclusion, a sonosensitizer-loaded multi-functional contrast agent was constructed and the feasibility was demonstrated, which might provide a novel strategy for tumour imaging and therapy.
本研究旨在开发一种载有声敏剂的多功能超声（US）造影剂，用于肿瘤治疗和成像。制备了血卟啉（HP）包封的聚乳酸-共-羟基乙酸（PLGA）微泡（HP-PLGA-MBs），并填充了全氟碳气体。通过体外和体内实验评估了 US 成像增强及其声动力学抗肿瘤效果。HP-PLGA-MBs 具有窄的粒径分布和光滑的表面，平均直径为 702.6±56.8nm，HP 包封效率为 63.50±1.26%，载药效率为 2.15±0.13%。HP-PLGA-MBs 可很好地增强体外和体内的超声成像。HP-PLGA-MBs 介导的声动力学治疗可获得显著的抗肿瘤效果。在 HP-PLGA-MBs 加超声组中，肿瘤生长率和肿瘤增殖指数最低，而在 HP-PLGA-MBs 加超声组中，肿瘤细胞凋亡指数最大。总之，构建了一种载有声敏剂的多功能造影剂，并验证了其可行性，这可能为肿瘤成像和治疗提供一种新策略。
