焦磷酸测序法检测和区分人血样本中的疟原虫（恶性疟原虫和间日疟原虫）的分子标记物
Molecular markers for detection and differentiation of Plasmodium falciparum and Plasmodium vivax in human blood samples by pyrosequencing.
机构信息
Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand.
出版信息
J Clin Microbiol. 2012 Apr;50(4):1455-7. doi: 10.1128/JCM.06001-11. Epub 2012 Feb 1.
Abstract
PCR amplification coupled with pyrosequencing was used to measure molecular markers that could be used to detect and differentiate Plasmodium falciparum and Plasmodium vivax in human blood samples. The detection rates were in agreement with the results of Giemsa-stained film microscopy, which is the current gold standard for detection. This method provides an exciting alternative for malaria diagnosis.
摘要
聚合酶链反应（PCR）扩增结合焦磷酸测序用于测量分子标记物，这些标记物可用于检测和区分人类血液样本中的恶性疟原虫和间日疟原虫。检测率与吉姆萨染色涂片显微镜检查的结果一致，后者是目前检测的金标准。这种方法为疟疾诊断提供了一种令人兴奋的替代方法。
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