School of Life Sciences, Institute of Bioengineering and Laboratory of Stem Cell Bioengineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Curr Opin Cell Biol. 2012 Apr;24(2):236-44. doi: 10.1016/j.ceb.2012.01.006. Epub 2012 Jan 31.
The complexity of stem cell niches poses a tremendous challenge to understanding mechanisms of extrinsic regulation of stem cell fate. In order to better understand niche signaling and its effect on stem cell fate choices, in vitro systems are being engineered which recapitulate, in a simplistic but increasingly sophisticated manner, native stem cell niches. New technologies or new combinations of existing technologies allow more systematic ways to probe niche signaling in high-throughput. Systems biology approaches in experimental design, data acquisition and analysis will be necessary to tackle the challenges that lie ahead.
干细胞龛的复杂性给理解干细胞命运的外在调控机制带来了巨大挑战。为了更好地理解龛信号及其对干细胞命运选择的影响，人们正在构建体外系统，以简单但越来越复杂的方式再现天然的干细胞龛。新技术或现有技术的新组合允许更系统地在高通量水平探测龛信号。在实验设计、数据获取和分析方面的系统生物学方法将是解决未来挑战所必需的。
