亲和免疫印迹法检测与糖鞘脂反应的血清单克隆免疫球蛋白
Affinity immunoblotting detection of serum monoclonal immunoglobulins reactive with glycosphingolipids.
作者信息
Jauberteau M O, Cook J M, Drouet M, Preud'homme J L
机构信息
Laboratory of Immunology, University Hospital, Limoges, France.
出版信息
J Immunol Methods. 1990 Nov 6;134(1):107-12. doi: 10.1016/0022-1759(90)90118-f.
The characterization of serum monoclonal IgM reactive with glycosphingolipid present in the nervous system is of major importance in patients with certain neuropathies. A rapid, sensitive and specific one-step method which permits such a characterization is described. Serum immunoglobulins separated by high resolution agarose electrophoresis are transferred to affinity filters (nitrocellulose sheets coated with glycolipids), and revealed with enzyme-tagged monospecific anti-immunoglobulin antibodies.
对于某些神经病变患者而言，鉴定与神经系统中存在的糖鞘脂发生反应的血清单克隆IgM至关重要。本文描述了一种快速、灵敏且特异的一步法，可用于此类鉴定。通过高分辨率琼脂糖电泳分离的血清免疫球蛋白被转移至亲和滤膜（涂有糖脂的硝酸纤维素膜）上，并用酶标记的单特异性抗免疫球蛋白抗体进行显色。
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