基于色烯喹啉的荧光开启型硫醇探针用于生物成像。
A chromenoquinoline-based fluorescent off-on thiol probe for bioimaging.
机构信息
Department of Chemistry, Dmitri Mendeleev Block, Indian Institute of Science Education and Research Pune, India.
出版信息
Chem Commun (Camb). 2012 Mar 11;48(21):2722-4. doi: 10.1039/c2cc16593g. Epub 2012 Feb 3.
PMID:22301487
Abstract
A new chromenoquinoline-based fluorescent off-on thiol probe 2 is reported. In aqueous buffer solutions at physiological pH, the probe exhibited 223-fold enhancement in fluorescence intensity by a Michael addition of cysteine to the maleimide appended to a chromenoquinoline. Cell permeability and live cell imaging of thiols are also demonstrated.
摘要
报道了一种新型基于色烯并喹啉的荧光“关-开”型硫醇探针 2。在生理 pH 的水性缓冲溶液中，通过半胱氨酸对连接在色烯并喹啉上的马来酰亚胺的迈克尔加成反应，探针的荧光强度增强了 223 倍。还证明了该探针具有良好的细胞通透性和对硫醇的活细胞成像能力。
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