用于选择性检测半胱氨酸/同型半胱氨酸的新型荧光探针
A New Turn on Fluorescent Probe for Selective Detection of Cysteine/Homocysteine.
作者信息
Xie Puhui, Gao Guangqin, Liu Juanjuan, Jin Qiu, Yang Guoyu
机构信息
College of Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
出版信息
J Fluoresc. 2015 Sep;25(5):1315-21. doi: 10.1007/s10895-015-1619-9. Epub 2015 Jul 17.
A new fluorescent probe (probe 1) was developed for recognition of cysteine (Cys) and homocysteine (Hcy). Probe 1 exhibited a large absorption peak blue-shift (107 nm) as well as enhanced fluorescence responses to Cys/Hcy based on cyclization of thiol containing amino acids with aldehydes, inhibiting the C = N isomerization-induced quenching process by an intramolecular hydrogen bond. The detection mechanism was proved by (1)H NMR and mass spectrometry analysis.
开发了一种用于识别半胱氨酸（Cys）和同型半胱氨酸（Hcy）的新型荧光探针（探针1）。基于含硫醇氨基酸与醛的环化反应，探针1表现出较大的吸收峰蓝移（107 nm）以及对Cys/Hcy增强的荧光响应，通过分子内氢键抑制C=N异构化诱导的猝灭过程。通过核磁共振氢谱（¹H NMR）和质谱分析证明了检测机制。
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