Olsen Douglas P, Keshishian Haig
Cold Spring Harb Protoc. 2012 Feb 1;2012(2):162-73. doi: 10.1101/pdb.top067785.
The Drosophila neuromuscular junction (NMJ) ranks as one of the preeminent model systems for studying synaptic development, function, and plasticity. In this article, we review the experimental genetic methods that include the use of mutated or reengineered ion channels to manipulate the synaptic connections made by motor neurons onto larval body-wall muscles. We also provide a consideration of environmental and rearing conditions that phenocopy some of the genetic manipulations.
果蝇神经肌肉接头（NMJ）是研究突触发育、功能和可塑性的卓越模型系统之一。在本文中，我们综述了实验遗传学方法，这些方法包括使用突变或重新设计的离子通道来操纵运动神经元与幼虫体壁肌肉之间形成的突触连接。我们还考虑了模拟某些基因操作的环境和饲养条件。
