Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei, People's Republic of China.
Am J Bot. 2012 Feb;99(2):e78-80. doi: 10.3732/ajb.1100365. Epub 2012 Feb 1.
The first microsatellite primers were developed for Davidia involucrata, an endangered relic species of the Tertiary in China, to further describe its genetic variability and population structure.
Using the Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO) protocol, 15 polymorphic microsatellite loci were isolated and characterized in 20 individuals from the germplasm collections of D. involucrata at the Hunan Forest Botanical Garden. High levels of polymorphism were revealed, with the total number of alleles per locus and the number of alleles per locus per individual ranging from two to 13 and from one to six, respectively.
The multibanded patterns of microsatellite loci obtained in the current study confirmed that D. involucrata might be a polyploid species. The primers will be useful for studies of genetic diversity and for guiding conservation strategies for D. involucrata.
首次为中国第三纪孑遗濒危物种珙桐开发微卫星引物，以进一步描述其遗传变异性和种群结构。
利用含重复序列的快速 AFLP 分离（FIASCO）方案，从湖南森林植物园的珙桐种质资源中 20 个个体中分离和鉴定出 15 个多态性微卫星位点。揭示了高水平的多态性，每个位点的等位基因总数和每个个体的每个位点的等位基因数分别从 2 到 13 和 1 到 6 不等。
本研究中获得的微卫星位点的多带模式证实，珙桐可能是一个多倍体物种。这些引物将有助于研究遗传多样性，并为珙桐的保护策略提供指导。
Zotero 插件