Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences, Seoul, Korea.
J Radiat Res. 2012;53(1):79-86. doi: 10.1269/jrr.11049.
The aim of this study was to determine whether extremely low frequency magnetic fields (ELF-MF) could affect intracellular reactive oxygen species (ROS) levels and antioxidant enzyme activity. After MCF10A human breast epithelial cells were exposed to 1 mT of 60 Hz ELF-MF for 4 hours, intracellular ROS level, superoxide dismutase (SOD) activity, and reduced to oxidized glutathione (GSH/GSSG) ratio were measured. The cells exposed to ELF-MF did not evidence statistically significant changes in the above-mentioned biological parameters as compared to either the incubator controls or sham-exposed cells. By way of contrast, the IR-exposed cells exhibited marked changes in ROS level, SOD activity, and GSH/GSSG ratio. When we assessed morphological changes and senescence-associated beta-galactosidase (SA-β-Gal) activity, only the IR-exposed cells were positive. According to our results, it could be concluded that ELF-MF has no effect on intracellular ROS level, SOD activity, and GSH/GSSG ratio under our exposure condition.
本研究旨在确定极低频磁场（ELF-MF）是否会影响细胞内活性氧（ROS）水平和抗氧化酶活性。将 MCF10A 人乳腺上皮细胞暴露于 1 mT、60 Hz 的 ELF-MF 中 4 小时后，测量细胞内 ROS 水平、超氧化物歧化酶（SOD）活性和还原型谷胱甘肽（GSH）/氧化型谷胱甘肽（GSSG）比值。与孵育对照或假暴露细胞相比，暴露于 ELF-MF 的细胞在上述生物学参数方面没有统计学上的显著变化。相比之下，IR 暴露的细胞表现出 ROS 水平、SOD 活性和 GSH/GSSG 比值的明显变化。当我们评估形态变化和衰老相关的β-半乳糖苷酶（SA-β-Gal）活性时，只有 IR 暴露的细胞呈阳性。根据我们的结果，可以得出结论，在我们的暴露条件下，ELF-MF 对细胞内 ROS 水平、SOD 活性和 GSH/GSSG 比值没有影响。
