Department of Radiology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan.
Jpn J Radiol. 2012 May;30(4):323-30. doi: 10.1007/s11604-012-0054-y.
To determine cellular viability of lung parenchyma and neoplastic cells in areas of ground-glass opacity (GGO) on computed tomography (CT) images immediately after pulmonary radiofrequency ablation (RFA) in rabbits.
A LeVeen RFA electrode was placed percutaneously into rabbit lungs with or without metastatic VX2 tumors. Five minutes later, seven isolated lungs were imaged by use of a multi-detector row CT scanner, and the images were compared with histological features. The cellular viability of the lung tissues was assessed by nicotinamide adenine dinucleotide hydrogen (NADH) staining in eight normal lungs and in three lungs with multiple metastatic tumors.
All lung lesions appeared as bilayered structures with a central, dense, attenuated area and an outer area of GGO on CT images, and as three-layered structures on macroscopic and microscopic images 5 min after RFA. The GGO areas approximately corresponded to the outer two layers in macroscopic images that were exudative and congestive on microscopic images. Staining for NADH was significantly reduced in the GGO and densely attenuated areas with or without tumor tissue staining compared with the non-ablated area.
Our results suggest that an area of GGO that appears on CT immediately after RFA can be effectively treated by RFA.
在兔肺部经皮放置 LeVeen 射频消融 (RFA) 电极后即刻，通过计算体层摄影术 (CT) 图像确定磨玻璃密度 (GGO) 区域的肺实质和肿瘤细胞的细胞活力。
在有或无转移性 VX2 肿瘤的兔肺中，经皮放置 LeVeen RFA 电极。5 分钟后，使用多排 CT 扫描仪对 7 个孤立的肺进行成像，并将图像与组织学特征进行比较。通过对 8 个正常肺和 3 个有多发性转移性肿瘤的肺进行烟酰胺腺嘌呤二核苷酸氢 (NADH) 染色，评估肺组织的细胞活力。
所有的肺病变在 CT 图像上均显示为双层结构，中央有一密集衰减区和外围的 GGO 区，RFA 后 5 分钟在大体和显微镜图像上表现为三层结构。GGO 区与大体图像上的外两层相对应，显微镜下表现为渗出性和充血性。与未消融区相比，GGO 和密集衰减区的 NADH 染色显著减少，无论是否有肿瘤组织染色。
我们的研究结果表明，RFA 后即刻 CT 上出现的 GGO 区域可通过 RFA 得到有效治疗。
