Department of Radiology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan,
Jpn J Radiol. 2014 Mar;32(3):145-54. doi: 10.1007/s11604-014-0282-4. Epub 2014 Jan 22.
The purpose of this study was to determine the effect of a synthetic tryptophan metabolite, tranilast [N-(3,4-dimethoxycinnamoyl)-anthranilic acid], on inflammatory and hemorrhagic areas after pulmonary radiofrequency ablation (RFA) in rabbits.
Percutaneous RFA using a 17-gauge LeVeen electrode was performed in normal rabbit lungs. The rabbits were divided into tranilast-treated (300 mg/kg/day, orally) and control groups (n = 24/group). The effects of tranilast were evaluated using multidetector-row computed tomography (CT), histology, and immunohistochemistry immediately after RFA on days 1, 7, 14, and 28.
Oral administration of tranilast significantly reduced the size of ablated lesions assessed using CT and histology on days 7 and 14. Furthermore, it reduced the hemorrhagic areas on day 7 and inflammatory areas on day 14, but did not affect the areas of coagulation necrosis on days 1, 7, 14, and 28. Immunohistochemical analysis showed an increase in the ratio of CD163-positive macrophage areas to rabbit macrophage (RAM11)-positive pan-macrophage areas and a decrease in the number of nuclear factor-κB-positive nuclei and CD31-positive microvessels in the tranilast group on days 7 and/or 14.
The results suggest that tranilast modulates the repair process after pulmonary RFA through macrophage accumulation, suppression of inflammation, and angiogenesis.
本研究旨在确定合成色氨酸代谢物曲尼司特[N-(3,4-二甲氧基肉桂酰)-邻氨基苯甲酸]对兔肺射频消融（RFA）后炎症和出血区域的影响。
在正常兔肺中经皮使用 17 号 LeVeen 电极进行 RFA。将兔子分为曲尼司特治疗组（300mg/kg/天，口服）和对照组（每组 24 只）。在 RFA 后即刻、第 1、7、14 和 28 天，使用多排 CT、组织学和免疫组织化学评估曲尼司特的作用。
曲尼司特的口服给药显著减小了 CT 和组织学评估的消融病变大小在第 7 和 14 天。此外，它减少了第 7 天的出血区域和第 14 天的炎症区域，但对第 1、7、14 和 28 天的凝血坏死区域没有影响。免疫组织化学分析显示，在第 7 和/或 14 天，曲尼司特组的 CD163 阳性巨噬细胞区域与兔巨噬细胞（RAM11）阳性全巨噬细胞区域的比例增加，核因子-κB 阳性核和 CD31 阳性微血管的数量减少。
结果表明，曲尼司特通过巨噬细胞积聚、炎症抑制和血管生成来调节肺 RFA 后的修复过程。
