Predicting kinase substrates using conservation of local motif density.

MOTIVATION

Protein kinases represent critical links in cell signaling. A central problem in computational biology is to systematically identify their substrates.

RESULTS

This study introduces a new method to predict kinase substrates by extracting evolutionary information from multiple sequence alignments in a manner that is tolerant to degenerate motif positioning. Given a known consensus, the new method (ConDens) compares the observed density of matches to a null model of evolution and does not require labeled training data. We confirmed that ConDens has improved performance compared with several existing methods in the field. Further, we show that it is generalizable and can predict interesting substrates for several important eukaryotic kinases where training data is not available.

AVAILABILITY AND IMPLEMENTATION

ConDens can be found at http://www.moseslab.csb.utoronto.ca/andyl/.

CONTACT

alan.moses@utoronto.ca

SUPPLEMENTARY INFORMATION

Supplementary data are available at Bioinformatics online.