Henares Terence G, Mizutani Fumio, Hisamoto Hideaki
Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297 Japan.
Anal Chim Acta. 2008 Mar 17;611(1):17-30. doi: 10.1016/j.aca.2008.01.064. Epub 2008 Feb 5.
This review accounts for the current development in microfluidic immunosensing chips. The basic knowledge of immunoassay in relation to its microfluidic material substrate, fluid handling and detection mode are briefly discussed. Here, we mainly focused on the surface modification, antibody immobilization, detection, signal enhancement and multiple analyte sensing. Some of the clinically important currently implemented on the microfluidic immunoassay chips are C-reactive protein (CRP), prostate specific antigen (PSA), ferritin, vascular endothelial growth factor (VEGF), myoglobin (Myo), cardiac troponin T (cTnT), cardiac troponin I (cTnI), and creatine kinase-cardiac muscle isoform (CK-MB). The emerging microfludic immunosensor technology may be a promising prospect that can propel the improvement of clinical and medical diagnosis.
本综述阐述了微流控免疫传感芯片的当前发展情况。简要讨论了免疫测定的基础知识，涉及其微流控材料基质、流体处理和检测模式。在此，我们主要关注表面修饰、抗体固定、检测、信号增强和多分析物传感。目前在微流控免疫测定芯片上实现的一些临床上重要的物质包括C反应蛋白（CRP）、前列腺特异性抗原（PSA）、铁蛋白、血管内皮生长因子（VEGF）、肌红蛋白（Myo）、心肌肌钙蛋白T（cTnT）、心肌肌钙蛋白I（cTnI）和肌酸激酶心肌同工酶（CK-MB）。新兴的微流控免疫传感器技术可能是一个有前景的发展方向，能够推动临床和医学诊断的进步。
