Sugiyama M, Kojima M, Yasuda Y, Hori Y, Nishiyama Y, Sugiyama A, Kido N, Ota M
Department of Obstetrics and Gynecology, Meijo Hospital.
Kansenshogaku Zasshi. 1990 Jul;64(7):840-6. doi: 10.11150/kansenshogakuzasshi1970.64.840.
The type-specific polysaccharide antigen of the group B streptococcus (GBS) type Ia as extracted and purified according to the procedures of Kane and Karakawa. Using this purified polysaccharide antigen, we made a sensitive and specific assay system of enzyme-linked immunosorbent assay (ELISA) and measured the titres of of the type-specific antibodies in maternal sera and cord blood sera. The titres of antibodies in 78 pregnant women (26 Ia carriers, 18 other types of GBS carriers and 34 non carriers) were compared. A mother of an infant affected by early onset infection of GBS Ia had a titre of antibody 1:10 at delivery, while 2 years later she became a non carrier and had a titre of antibody over 1:160. The titres of antibodies in 27 pair sera of mothers and cords were well correlated.
按照凯恩（Kane）和卡拉川（Karakawa）的方法提取并纯化的B族链球菌（GBS）Ia型的型特异性多糖抗原。使用这种纯化的多糖抗原，我们建立了一种灵敏且特异的酶联免疫吸附测定（ELISA）检测系统，并测定了母体血清和脐带血血清中型特异性抗体的滴度。比较了78名孕妇（26名Ia型携带者、18名其他类型的GBS携带者和34名非携带者）的抗体滴度。一名婴儿受到GBS Ia型早发感染的母亲在分娩时抗体滴度为1:10，而两年后她成为非携带者，抗体滴度超过1:160。27对母婴血清中的抗体滴度具有良好的相关性。
