College of Biological Engineering, The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University, Xiamen, Fujian, China.
J Agric Food Chem. 2012 Mar 7;60(9):2190-9. doi: 10.1021/jf203779w. Epub 2012 Feb 22.
Arginine kinase (AK) is an important enzyme participating in energy metabolism in invertebrates, but, to date, there have been no reports that AK from octopus is an allergen. In this study, octopus AK was purified, and its molecular biological, immunological, and physicochemical characterizations were analyzed. The results showed that octopus AK was purified and confirmed by mass spectrometry for the first time, and its molecular mass was 38 kDa. The full-length gene sequence of octopus AK encompassed 1209 bp and was predicted to encode a protein with 348 amino acid residues. The homology of octopus AK and crustacean AK was about 54%, but the similarity between their three-dimensional structures was high. Octopus AK could react with mouse anti-shrimp AK and rabbit anti-crab AK polyclonal antibody singly. Octopus AK could also react with specific IgE of the sera from octopus-allergic patients effectively, whereas crab AK could inhibit the reaction between them. Finally, the IgE-binding activity of octopus AK could be reduced in the processes of thermal or acid-alkali treatment. In summary, AK was identified as a novel allergen in octopus, which had a sensitizing ability similar to that of crustacean AK. This is significant in allergy diagnosis and the treatment of octopus-allergic disorders.
精氨酸激酶（AK）是参与无脊椎动物能量代谢的重要酶，但迄今为止，尚无报道表明章鱼 AK 是过敏原。在本研究中，我们纯化了章鱼 AK，并对其进行了分子生物学、免疫学和物理化学特性分析。结果表明，我们首次通过质谱法纯化和确证了章鱼 AK，其分子量为 38 kDa。章鱼 AK 的全长基因序列包含 1209 个碱基对，预测编码一个由 348 个氨基酸残基组成的蛋白质。章鱼 AK 与甲壳类动物 AK 的同源性约为 54%，但其三维结构的相似性较高。章鱼 AK 可与单克隆抗虾 AK 抗体和兔抗蟹 AK 多克隆抗体发生反应。章鱼 AK 还可以与来自章鱼过敏患者的特异性 IgE 有效反应，而蟹 AK 可以抑制它们之间的反应。最后，章鱼 AK 的 IgE 结合活性可以在热或酸碱处理过程中降低。综上所述，AK 被鉴定为章鱼中的一种新型过敏原，其致敏能力与甲壳类动物 AK 相似。这对于过敏症诊断和治疗章鱼过敏症具有重要意义。
