State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, People's Republic of China.
Inorg Chem. 2012 Feb 20;51(4):2454-60. doi: 10.1021/ic2024082. Epub 2012 Feb 3.
A novel selective fluorescent chemosensor based on an 8-hydroxyquinoline-appended fluorescein derivative (L1) was synthesized and characterized. Once combined with Cu(2+), it displayed high specificity for sulfide anion. Among the various anions, only sulfide anion induced the revival of fluoresecence of L1, which was quenched by Cu(2+), resulting in "off-on"-type sensing of sulfide anion. What's more, the sensor was retrievable to indicate sulfide anions with Cu(2+), and S(2-), in turn, increased. With the addition of Cu(2+), compound L1 could give rise to a visible pink-to-yellow color change and green fluorescence quenching. The resulting yellow solution could change to pink and regenerate to green fluorescence immediately upon the addition of sulfide anion; however, no changes were observed in the presence of other anions, including CN(-), P(2)O(7)(4-), and other forms of sulfate, making compound L1 an extremely selective and efficient sulfide chemosensor. The signal transduction occurs via reversible formation-separation of complex L1Cu and CuS. What's more, the biological imaging study has demonstrated that the chemosensor can detect sulfur anions in biological systems at a relatively low concentration.
一种基于 8-羟基喹啉修饰的荧光素衍生物（L1）的新型选择性荧光化学传感器被合成并进行了表征。一旦与 Cu(2+) 结合，它对硫阴离子表现出高度的特异性。在各种阴离子中，只有硫阴离子能诱导 L1 的荧光恢复，而 L1 的荧光被 Cu(2+) 猝灭，从而实现对硫阴离子的“关-开”型传感。此外，该传感器可通过 Cu(2+) 来指示硫阴离子，并且 S(2-) 增加。随着 Cu(2+) 的加入，化合物 L1 会引起可见的粉红色到黄色的颜色变化和绿色荧光猝灭。加入硫阴离子后，生成的黄色溶液会立即变为粉红色并重新产生绿色荧光；然而，在存在其他阴离子，包括 CN(-)、P(2)O(7)(4-) 和其他形式的硫酸盐时，没有观察到任何变化，这使得化合物 L1 成为一种极其选择性和高效的硫阴离子化学传感器。信号转导是通过 L1Cu 和 CuS 的可逆形成和分离来实现的。此外，生物成像研究表明，该化学传感器可以在相对较低的浓度下检测生物体系中的硫阴离子。
