Nagase M, Udagawa E, Schumacher H R, Baker D G
Department of Orthopaedic Surgery, Gunma University School of Medicine, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1990 Jul;64(7):602-11.
The subcutaneous air pouch of the male Sprague-Dawley rat was used to study inflammatory potential of artificial ceramics. Local leukocyte influx, proteinase, prostaglandin E2 (PGE2) and tumor necrosis factor (TNF) levels were measured after injection of hydroxyapatite ceramic (HAP), tricalcium phosphate ceramic (TCP) and apatite-wollastonite glass ceramic (GC) into the pouch. Synthetic monosodium urate crystals (MSU) were used as positive controls and normal saline (NS) as a negative control. The response was monitored over a period of 168 hours by irrigating the pouch with 5 ml of NS and withdrawing 4 ml. MSU produced the greatest response in leukocyte counts, proteinase and PGE2, whereas HAP, TCP and GC often appeared later than with MSU. TNF was significantly detected only after ceramics and not after MSU. The chronic and relatively prolonged reaction to HAP, TCP and GC suggests that an inflammatory reaction may occur after implantation of these ceramics in humans. The increased levels of TNF and production of PGE2, which are substances proposed to contribute to osteolysis associated with loosening, are of special interest for further research.
采用雄性斯普拉格 - 道利大鼠的皮下气囊来研究人工陶瓷的炎症潜能。向气囊内注射羟基磷灰石陶瓷（HAP）、磷酸三钙陶瓷（TCP）和磷灰石 - 硅灰石玻璃陶瓷（GC）后，检测局部白细胞浸润、蛋白酶、前列腺素E2（PGE2）和肿瘤坏死因子（TNF）水平。合成尿酸钠晶体（MSU）用作阳性对照，生理盐水（NS）用作阴性对照。通过向气囊内注入5 ml NS并抽出4 ml，在168小时内监测反应情况。MSU在白细胞计数、蛋白酶和PGE2方面产生的反应最大，而HAP、TCP和GC的反应通常比MSU出现得晚。仅在注射陶瓷后检测到TNF，而注射MSU后未检测到。对HAP、TCP和GC的慢性且相对持久的反应表明，这些陶瓷植入人体后可能会发生炎症反应。TNF水平升高以及PGE2的产生，这些物质被认为与松动相关的骨溶解有关，值得进一步研究。
