Lazarus M D, Cuckler J M, Schumacher H R, Ducheyne P, Baker D G
Department of Orthopaedic Surgery, University of Pennsylvania School of Medicine, Philadelphia.
J Orthop Res. 1994 Jul;12(4):532-41. doi: 10.1002/jor.1100120410.
Particulate polymethylmethacrylate debris has been implicated in the inflammatory response observed surrounding loosened cemented implants. The rat subcutaneous pouch model and the Howie implant model (used to study bone resorption) were used to quantify the response to mechanically produced endotoxin-free polymethylmethacrylate debris with and without 10% (wt/vol) BaSO4. In the rat subcutaneous pouch model, the inflammatory response to polymethylmethacrylate particles containing BaSO4 was greater than the response to plain polymethylmethacrylate particles of similar size. Increased inflammation was measured by leukocyte counts and levels of prostaglandin E2, tumor necrosis factor, and neutral metalloprotease. In addition, particulate polymethylmethacrylate with BaSO4 caused significantly greater bone resorption in the Howie model than did particulate plain polymethylmethacrylate. In in vitro studies, particulate polymethylmethacrylate with BaSO4 stimulated more prostaglandin E2, neutral metalloprotease, and tumor necrosis factor from human monocytes in culture and stimulated greater proliferation of synovial cells than did particulate plain polymethylmethacrylate. The presence of BaSO4 appears to significantly intensify the inflammatory response to polymethylmethacrylate debris.
聚甲基丙烯酸甲酯颗粒碎片与在松动的骨水泥植入物周围观察到的炎症反应有关。大鼠皮下袋模型和豪伊植入模型（用于研究骨吸收）被用来量化对机械产生的不含内毒素的聚甲基丙烯酸甲酯碎片（有和没有10%（重量/体积）硫酸钡）的反应。在大鼠皮下袋模型中，对含硫酸钡的聚甲基丙烯酸甲酯颗粒的炎症反应大于对类似大小的普通聚甲基丙烯酸甲酯颗粒的反应。通过白细胞计数以及前列腺素E2、肿瘤坏死因子和中性金属蛋白酶的水平来测量炎症的增加。此外，在豪伊模型中，含硫酸钡的聚甲基丙烯酸甲酯颗粒比普通聚甲基丙烯酸甲酯颗粒引起的骨吸收明显更多。在体外研究中，含硫酸钡的聚甲基丙烯酸甲酯颗粒比普通聚甲基丙烯酸甲酯颗粒在培养中从人单核细胞刺激产生更多的前列腺素E2、中性金属蛋白酶和肿瘤坏死因子，并且刺激滑膜细胞的增殖更多。硫酸钡的存在似乎显著增强了对聚甲基丙烯酸甲酯碎片的炎症反应。
