Souiden Yousra, Mahdouani Manel, Chaieb Kamel, Bakhrouf Amina, Mahdouani Kacem
Laboratory of Molecular Biology, Hospital of Ibn Eljazzar of Kairouan, Avenue Ibn Eljazzar, Kairouan, Tunisia.
Genet Test Mol Biomarkers. 2012 Jul;16(7):661-6. doi: 10.1089/gtmb.2011.0212. Epub 2012 Feb 3.
Cytochrome P450 (CYP)1A1 gene polymorphism has been shown to be associated with several diseases. In this study, we evaluated the association between the polymorphism in the cytochrome P-450 (CYP)1A1 (CYP1A1) gene and genetic susceptibility to prostate cancer (PCa) in Tunisian men. One hundred and thirty eight PCa patients and the same number of controls were enrolled in this study. All DNA samples from peripheral blood leucocytes were genotyped for genetic polymorphism of the CYP1A1 gene using the polymerase chain reaction-restriction fragment length polymorphism method. The polymorphism in PCa patients was also analyzed according to age at diagnosis, tobacco use, cancer stage, and grade (Gleason score). The prevalence of CYP1A1 variants (w1m1 and m1m1) was similar in PCa patients and controls (15.22% vs. 17.39%, p=0.624 and 2.17%, respectively). No significant difference in the frequency distribution of CYP1A1 polymorphism was observed between PCa patients and controls. Furthermore, we were unable to demonstrate any significant association between the studied CYP1A1 polymorphism, age, tobacco use, and tumor parameters of aggressiveness at diagnosis.
细胞色素P450（CYP）1A1基因多态性已被证明与多种疾病有关。在本研究中，我们评估了突尼斯男性细胞色素P - 450（CYP）1A1（CYP1A1）基因多态性与前列腺癌（PCa）遗传易感性之间的关联。本研究纳入了138例PCa患者和相同数量的对照。使用聚合酶链反应 - 限制性片段长度多态性方法对所有外周血白细胞的DNA样本进行CYP1A1基因遗传多态性基因分型。还根据诊断年龄、吸烟情况、癌症分期和分级（ Gleason评分）对PCa患者的多态性进行了分析。CYP1A1变体（w1m1和m1m1）在PCa患者和对照中的患病率相似（分别为15.22%对17.39%，p = 0.624和2.17%）。在PCa患者和对照之间未观察到CYP1A1多态性频率分布的显著差异。此外，我们无法证明所研究的CYP1A1多态性、年龄、吸烟情况与诊断时侵袭性肿瘤参数之间存在任何显著关联。
