Division of Pediatrics, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
Ultrasound Med Biol. 2012 Mar;38(3):423-31. doi: 10.1016/j.ultrasmedbio.2011.12.003.
Cerebral intraventricular haemorrhage (IVH) is the most common cause of severe neurologic impairment following preterm birth in human infants. Ideally, an animal model for cerebral IVH should allow for reliable noninvasive evaluation of haemorrhagic extension and of subsequent development of posthaemorrhagic ventricular dilatation (PHVD). The aim of this study was to evaluate the use of high-frequency ultrasound (HFU) in premature rabbit pups with cerebral IVH induced by IP glycerol injection. Serial examinations using HFU enabled an accurate description of haemorrhagic extension and measurement of progressive PHVD over 72 h. The coefficient of variation for inter- and intraobserver variability in two measurements of ventricular size was less than 8.8% and 9.3%, respectively. Repeated ultrasound-guided intraventricular injection and sampling could be performed in vivo excluding requirement of stereotactic procedures and sedation. Application of HFU is a powerful tool for the evaluation of mechanisms involved in cerebral IVH and PHVD in the preterm rabbit pup model.
脑室内出血（IVH）是人类早产儿出生后严重神经功能障碍的最常见原因。理想情况下，脑 IVH 的动物模型应允许可靠地进行非侵入性评估，以评估出血的扩展情况以及随后发生的出血后脑室扩张（PHVD）。本研究旨在评估高频超声（HFU）在通过 IP 甘油注射诱导脑 IVH 的早产兔幼仔中的应用。使用 HFU 进行的连续检查可以准确描述出血的扩展，并在 72 小时内测量进行性 PHVD。两次测量脑室大小的观察者内和观察者间变异系数分别小于 8.8%和 9.3%。可以在体内重复进行超声引导的脑室内注射和取样，而无需进行立体定向手术和镇静。HFU 的应用是评估早产兔幼仔脑 IVH 和 PHVD 中涉及的机制的有力工具。
