苦龙芽 Vogel 提取物的体内外安全性评价。
In vitro and in vivo safety evaluation of Dipteryx alata Vogel extract.
机构信息
School of Pharmacy, University of Sorocaba, UNISO, Rodovia Raposo Tavares km 92.5, Zip code: 18023-000 Sorocaba, SP, Brazil.
出版信息
BMC Complement Altern Med. 2012 Feb 3;12:9. doi: 10.1186/1472-6882-12-9.
BACKGROUND
Dipteryx alata Vogel popularly known as "baru" is an important commercial leguminous tree species from the Brazilian Cerrado, which possess medicinal properties, besides its fruits consumption by animals and humans. The use of the "naturally occurring plants" as herbal remedies and foods mainly from leaves, seeds, flowers and roots of plants or extracts require precautions before ensuring these are safe and efficacious. The objective of this study was to evaluate the safety of D. alata barks extract.
METHODS
Vegetal drugs of D. alata barks were submitted to quality control assays and further to the safety assays under 1) in vitro parameter by Salmonella (Ames) mutagenicity, and 2) in vivo parameter on the pregnancy of rats.
RESULTS
The extract was non-mutagenic to any of the assessed strains TA97a, TA98, TA100 and TA102 even after metabolic activation (+S9). All in vivo parameters (reproductive ability evaluation, physical development of rat offsprings, and neurobehavioral development assays) showed no changes related to control group.
CONCLUSION
D. alata barks extract is neither mutagenic by the Ames test nor toxic in the pregnancy of rats, with no physical-neurobehavioral consequences on the rat offsprings development.
背景
双翅目的 Vogel 俗称“baru”是来自巴西塞拉多的一种重要的商业豆科树种，除了其果实被动物和人类食用外，还具有药用特性。“天然植物”作为草药和食物的使用，主要来自植物的叶子、种子、花朵和根或提取物，在确保这些植物安全和有效之前需要谨慎。本研究的目的是评估 D. alata 树皮提取物的安全性。
方法
D. alata 树皮的植物药经过质量控制检测，然后进一步进行安全性检测，包括 1）体外参数沙门氏菌（Ames）致突变性，和 2）在大鼠怀孕的体内参数。
结果
提取物对评估的 TA97a、TA98、TA100 和 TA102 菌株均无致突变性，即使在代谢激活（+S9）后也是如此。所有体内参数（生殖能力评估、大鼠后代的身体发育和神经行为发育测定）均未显示与对照组相关的变化。
结论
D. alata 树皮提取物既没有通过 Ames 试验产生致突变性，也没有在大鼠怀孕时产生毒性，对大鼠后代的发育没有身体神经行为方面的影响。
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