Department of Chemical Engineering, Institute of Chemical Technology, University of Mumbai,(1) Matunga, Mumbai 400 019, India.
Bioresour Technol. 2012 Apr;109:1-6. doi: 10.1016/j.biortech.2012.01.030. Epub 2012 Jan 16.
Lipase catalyzed transesterification was investigated to study the synergistic effect of microwave irradiation and enzyme catalysis. Transesterification of ethyl-3-phenylpropanoate with n-butanol was chosen as the model reaction using immobilized enzymes such as Novozyme 435, Lipozyme RMIM and Lipozyme TL IM with microwave irradiation. Novozyme 435 was the best catalyst. The effect of various parameters affecting the conversion and initial rates of transesterification were studied to establish kinetics and mechanism. There is synergism between enzyme catalysis and microwave irradiation. The analysis of initial rate data and progress curve data showed that the reaction obeys the Ping-Pong bi-bi mechanism with inhibition by n-butanol. The theoretical predictions and experimental data match very well. These studies were also extended to other alcohols such as 2-phenyl-1-propanol, n-octanol, benzyl alcohol, iso-amyl alcohol, 2-hexanol and 2-pentanol under otherwise similar conditions.
研究了脂肪酶催化的酯交换反应，以研究微波辐射和酶催化的协同效应。选择固定化酶（如 Novozyme 435、Lipozyme RMIM 和 Lipozyme TL IM）催化的 3-苯基丙酸乙酯与正丁醇的酯交换反应作为模型反应，并进行微波辐射。Novozyme 435 是最佳的催化剂。研究了影响转化率和初始反应速率的各种参数的影响，以建立动力学和机制。酶催化和微波辐射之间存在协同作用。初始速率数据和进度曲线数据的分析表明，该反应遵循乒乓双酶机制，被正丁醇抑制。理论预测和实验数据非常吻合。在其他类似条件下，这些研究也扩展到了其他醇类，如 2-苯基-1-丙醇、正辛醇、苯甲醇、异戊醇、2-己醇和 2-戊醇。
