Amini Yalda, Shahedi Mansour, Habibi Zohreh, Yousefi Maryam, Ashjari Maryam, Mohammadi Mehdi
Department of Organic Chemistry and Oil, Faculty of Chemistry, Shahid Beheshti University, Tehran, Iran.
Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Bioresour Bioprocess. 2022 May 30;9(1):60. doi: 10.1186/s40643-022-00552-0.
A new approach was used for the immobilization of Thermomyces lanuginosus lipase (TLL), Candida antarctica lipase B (CALB), and Rhizomucor miehei lipase (RML) on amine-functionalized magnetic nanoparticles (FeO@SiO-NH) via a multi-component reaction route (using cyclohexyl isocyanide). The used method offered a single-step and very fast process for covalent attachment of the lipases under extremely mild reaction conditions (25 °C, water, and pH 7.0). Rapid and simple immobilization of 20 mg of RML, TLL, and CALB on 1 g of the support produced 100%, 98.5%, and 99.2% immobilization yields, respectively, after 2 h of incubation. The immobilized derivatives were then used for biodiesel production from waste cooking oil. Response surface methodology (RSM) in combination with central composite rotatable design (CCRD) was employed to evaluate and optimize the biodiesel production. The effect of some parameters such as catalyst amount, reaction temperature, methanol concentration, water content for TLL or water-adsorbent for RML and CALB, and ratio of t-butanol (wt%) were investigated on the fatty acid methyl esters (FAME) yield.
通过多组分反应路线（使用环己基异氰化物），采用一种新方法将嗜热栖热菌脂肪酶（TLL）、南极假丝酵母脂肪酶B（CALB）和米黑根毛霉脂肪酶（RML）固定在胺功能化磁性纳米颗粒（FeO@SiO-NH）上。所采用的方法在极其温和的反应条件（25°C、水和pH 7.0）下，为脂肪酶的共价连接提供了一步且非常快速的过程。在1 g载体上快速简单地固定20 mg的RML、TLL和CALB，孵育2 h后，固定化产率分别为100%、98.5%和99.2%。然后将固定化衍生物用于从废食用油生产生物柴油。采用响应面法（RSM）结合中心复合旋转设计（CCRD）来评估和优化生物柴油生产。研究了一些参数，如催化剂用量、反应温度、甲醇浓度、TLL的含水量或RML和CALB的吸水剂以及叔丁醇比例（wt%）对脂肪酸甲酯（FAME）产率的影响。
